<p>Sugar beet (<i>Beta vulgaris</i> L.) is the second sugar crop grown in Egypt after sugarcane, and it contributes significantly to sugar production. Rust disease caused by <i>Uromyces betae</i> is among the numerous fungal diseases that affect sugar beet and it has a significant effect on productivity. The purpose of this study was to examine the impacts of thiourea (CH₄N₂S) and moringa (<i>Moringa oleifera</i>) seed oil treatments on disease control, root yield and sugar content. In vitro, thiourea (5, 10, 15 and 20&#xa0;mM) and moringa essential oil (100, 250, 500, 750 and 1000&#xa0;μl/L) were tested for their ability to inhibit the germination of urediniospores of <i>U. betae</i>. Thiourea significantly decreased the germination of urediniospores of <i>U. betae</i> at the concentrations studied, from 100% in the control to 26.0% at 20&#xa0;mM, and the germination was 63.0% to 29.3% with increasing concentration from 100 – 1000 µL L⁻¹ for moringa oil. Under greenhouse and field conditions, disease severity was significantly reduced with both treatments. In the greenhouse, these were lowered (%, mean ± SD) to 11.03 ± 1.44 and 11.12 ± 1.88 using moringa oil, and field trials (2023/24 and 2024/25 seasons,) of thiourea dipped tubers gave disease scores ranging from by (42.8–45.5% %) when compared with untreated control. Root yield and TSS increased significantly; the highest root yield (34.56 ± 3.11 t feddan⁻¹) and sugar content (21.01 ± 0.44%) were obtained with thiourea. They also reduced potassium, sodium, and α-amino-nitrogen. We could recommend moringa essential oil and thiourea as powerful,safe alternatives for managing sugar beet rust disease and improving root yield characteristics. Moringa oil is an effective antifungal agent against fungal plant diseases, but more thorough research is needed to understand its effects fully.</p>