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Background <p>Inflammatory bowel disease (IBD) is a chronic disease that includes Crohn’s disease and ulcerative colitis (UC). Tight junctions (TJs) play an important role in intestinal barrier function. In intestinal epithelial cells, TJs are composed of different junction molecules, including claudin and occludin (OCLN), and regulate the paracellular permeability of water, ions, and macromolecules in adjacent cells. The TJ structure also provides a physical barrier to luminal inflammatory molecules, although impaired integrity and structure of the TJ barrier can result in the forcible activation of immune cells and inflammation.</p> Objective <p>This study aimed to investigate the protective effects of <i>Angelica gigas Nakai</i> extracts (AGNE) on intestinal barrier and TJ activation.</p> Results <p>AGNE exhibited no cytotoxic activity against Caco-2 cells and showed potent antioxidant activity. Moreover, AGNE treatment significantly increased transepithelial electrical resistance (TEER) values and reduced epithelial permeability. In addition, the expression of Zonula Occludens (ZO)-1 and OCLN increased in a concentration-dependent manner with AGNE treatment. Conversely, myosin light chain kinase (MLCK) expression decreased in a concentration-dependent manner upon AGNE treatment. Notably, the most significant improvement was observed in the AGNE 200&#xa0;μg/mL group compared to the IL-6 control group.</p> Conclusion <p>Our findings highlight the potential usefulness of AGNE as a novel therapeutic agent for maintaining epithelial barrier integrity in IBD.</p>

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Angelica gigas Nakai hot water extracts alleviate IL-6-induced ulcerative colitis by restoring intestinal barrier function

  • 期刊论文

  • 发表日期：

    2025年4月10日

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  • Ye Jin Yang,
  • Min Jung Kim,
  • Ji Woong Heo,
  • Han Nim Choi,
  • Dongbin Lee,
  • Young Hun Kim,
  • Kwang Il Park

摘要

Background

Inflammatory bowel disease (IBD) is a chronic disease that includes Crohn’s disease and ulcerative colitis (UC). Tight junctions (TJs) play an important role in intestinal barrier function. In intestinal epithelial cells, TJs are composed of different junction molecules, including claudin and occludin (OCLN), and regulate the paracellular permeability of water, ions, and macromolecules in adjacent cells. The TJ structure also provides a physical barrier to luminal inflammatory molecules, although impaired integrity and structure of the TJ barrier can result in the forcible activation of immune cells and inflammation.

Objective

This study aimed to investigate the protective effects of Angelica gigas Nakai extracts (AGNE) on intestinal barrier and TJ activation.

Results

AGNE exhibited no cytotoxic activity against Caco-2 cells and showed potent antioxidant activity. Moreover, AGNE treatment significantly increased transepithelial electrical resistance (TEER) values and reduced epithelial permeability. In addition, the expression of Zonula Occludens (ZO)-1 and OCLN increased in a concentration-dependent manner with AGNE treatment. Conversely, myosin light chain kinase (MLCK) expression decreased in a concentration-dependent manner upon AGNE treatment. Notably, the most significant improvement was observed in the AGNE 200 μg/mL group compared to the IL-6 control group.

Conclusion

Our findings highlight the potential usefulness of AGNE as a novel therapeutic agent for maintaining epithelial barrier integrity in IBD.