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<p>The myosin light-chain kinase<b> (</b><i>MYLK4</i>) gene plays several vital functions in biological processes. Its role in muscle and other tissues is well studied in humans and mice, but less studied in case of lower vertebrates, especially fishes. This study was focussed to mine and characterize <i>MYLK4</i> gene from the <i>Clarias magur</i> (magur) transcriptome data, considering <i>C. magur</i>, <i>Ictalurus punctatus</i> and <i>Danio rerio</i> genomes as references. <i>MYLK4</i> gene of family member 4-like isoform X3 was used for primer&#xa0;designing and validation. Protein–protein interaction (PPI) analysis revealed interplay and co-expression of <i>MYLK4</i> gene with <i>phospholipase C beta 4</i>. A total of 53 SSRs were identified in the intronic region and 442 SNPs found in exonic as well as intronic regions. These SSRs and SNPs, identified in six individuals, could be used to study magur populations. The present finding on structural annotation, protein–protein interaction network and expression profile may be useful towards functional explanation of the gene and help to predict its role in growth of the catfishes. This article aligns with SDG 14 (Life Below Water) of the UN Agenda for Sustainable Development&#xa0;Goals.</p>

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Characterization and growth associated expression of myosin light-chain kinase gene in Indian catfish, Clarias magur

  • 期刊论文

  • 发表日期：

    2026年5月6日

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  • Vishwamitra Singh Baisvar,
  • Murali Sanjeev Kumar,
  • Basdeo Kushwaha,
  • Satyamvada Maurya,
  • Ajay Kumar Singh,
  • Uttam Kumar Sarkar,
  • Krishna Kumar Chaturvedi,
  • Neeraj Budhlakoti,
  • Anil Rai,
  • Ravindra Kumar

摘要

The myosin light-chain kinase (MYLK4) gene plays several vital functions in biological processes. Its role in muscle and other tissues is well studied in humans and mice, but less studied in case of lower vertebrates, especially fishes. This study was focussed to mine and characterize MYLK4 gene from the Clarias magur (magur) transcriptome data, considering C. magur, Ictalurus punctatus and Danio rerio genomes as references. MYLK4 gene of family member 4-like isoform X3 was used for primer designing and validation. Protein–protein interaction (PPI) analysis revealed interplay and co-expression of MYLK4 gene with phospholipase C beta 4. A total of 53 SSRs were identified in the intronic region and 442 SNPs found in exonic as well as intronic regions. These SSRs and SNPs, identified in six individuals, could be used to study magur populations. The present finding on structural annotation, protein–protein interaction network and expression profile may be useful towards functional explanation of the gene and help to predict its role in growth of the catfishes. This article aligns with SDG 14 (Life Below Water) of the UN Agenda for Sustainable Development Goals.