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<p>Sheath blight (ShB) caused by the necrotrophic fungus <i>Rhizoctonia solani,</i> is a major disease of rice, with no completely resistant genotype identified to date. In the present study, a major QTL, <i>qShB-1.1,</i> covering ~ 0.77 megabase (Mb) interval at the telomeric end of the long arm in chromosome 1, was consistently identified from a biparental mapping population of 384 recombinant inbred lines (RILs) derived from moderately resistant genotype CR 1014 and susceptible genotype Swarna-Sub1. Validation in contrasting RILs and backcross-derived inbred lines (BILs) through field-based phenotyping, glass house screenings, and bioassay confirmed a strong association between the flanking markers and ShB response. Colocalization of <i>qShB-1.1</i> identified the corresponding chromosomal location as a genomic hotspot for ShB resistance QTLs. Within <i>qShB-1.1</i>, 122 genes were annotated, out of which 17 were shortlisted based on their tissue-specific expressions reported in databases and literature. Six of these genes were directly identified from the differential proteomics dataset of the parents generated at different time intervals after inoculation. Three other genes,&#xa0;<i>LOC_Os01g65800</i>, <i>LOC_Os01g65650</i>, and <i>LOC_Os01g65450</i>, exhibited significantly higher expression &#xa0;as determined by quantitative real-time PCR (qRT-PCR) in all resistant genotypes. Cis-regulatory elements, including methyl jasmonate (MeJA) and other stress-responsive motifs, were identified in these genes, suggesting their putative role in ShB defense responses. Additionally, 98 co-expressed genes, for these three&#xa0;genes, were predicted and further verified in the differential proteomic dataset. The stable QTL <i>qShB-1.1</i> thus represents a valuable genomic resource that could be deployed in marker-assisted breeding and gene pyramiding to mitigate one of the most critical diseases of rice. This article aligns with SDG 2 (Zero Hunger) of the UN Agenda for Sustainable Development.</p>

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High-resolution mapping of stable sheath blight resistance QTL qShb-1.1 from rice cultivar CR 1014

  • 期刊论文

  • 发表日期：

    2026年5月21日

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  • Archana Bal,
  • Pankajini Samal,
  • Arup Kumar Mukherjee,
  • Lambodar Behera,
  • Mridul Chakraborti,
  • Kutubuddin A. Molla,
  • Meera Kumari Kar

摘要

Sheath blight (ShB) caused by the necrotrophic fungus Rhizoctonia solani, is a major disease of rice, with no completely resistant genotype identified to date. In the present study, a major QTL, qShB-1.1, covering ~ 0.77 megabase (Mb) interval at the telomeric end of the long arm in chromosome 1, was consistently identified from a biparental mapping population of 384 recombinant inbred lines (RILs) derived from moderately resistant genotype CR 1014 and susceptible genotype Swarna-Sub1. Validation in contrasting RILs and backcross-derived inbred lines (BILs) through field-based phenotyping, glass house screenings, and bioassay confirmed a strong association between the flanking markers and ShB response. Colocalization of qShB-1.1 identified the corresponding chromosomal location as a genomic hotspot for ShB resistance QTLs. Within qShB-1.1, 122 genes were annotated, out of which 17 were shortlisted based on their tissue-specific expressions reported in databases and literature. Six of these genes were directly identified from the differential proteomics dataset of the parents generated at different time intervals after inoculation. Three other genes, LOC_Os01g65800, LOC_Os01g65650, and LOC_Os01g65450, exhibited significantly higher expression  as determined by quantitative real-time PCR (qRT-PCR) in all resistant genotypes. Cis-regulatory elements, including methyl jasmonate (MeJA) and other stress-responsive motifs, were identified in these genes, suggesting their putative role in ShB defense responses. Additionally, 98 co-expressed genes, for these three genes, were predicted and further verified in the differential proteomic dataset. The stable QTL qShB-1.1 thus represents a valuable genomic resource that could be deployed in marker-assisted breeding and gene pyramiding to mitigate one of the most critical diseases of rice. This article aligns with SDG 2 (Zero Hunger) of the UN Agenda for Sustainable Development.