<p>Ornamental group of plants have been particularly promoted worldwide by classical breeding and induced mutation techniques. The latter has enabled development of numerous favored varieties. Classical mutation combined with tissue culture have led to realize complete package of mutation techniques to develop new varieties through genetic manipulations for vegetative propagated ornamentals. A wide range of advanced techniques (TILLING, DNA-based techniques, etc.) have been standardized, which claim to be effective promoting the existing characteristics prospecting more beneficial novel types. Each method, along with its supporting parameters, contributes logistically to the development of a new variety. For an improvement program, one should have up-to-date knowledge about technical details and their merits and demerits. Classical mutation technique is now well organized for almost all ornamental plants, but EMS-TILLING, EcoTILLING, NGS-TILLING promises added value. Whereas, gene editing opens new avenues, but its applications for ornamentals plants is still in its infancy. Breeders should be conscious about the pros and cons of differing perspective and should consciously select the plan which is logistically appropriate, along with budget, for achieving the target under the current situation of variety advancement. An attempt has been made in this review to evaluate the usefulness of all major techniques to substantiate mutation development in ornamentals. This article aligns with SDG 2 (Zero Hunger) of the UN Agenda for Sustainable Development.</p>

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Impact of classically induced mutations on ornamental plants and comparative assessment of advanced techniques (TILLING) for induction and detection of mutations and mutants

  • Subodh Kumar Datta

摘要

Ornamental group of plants have been particularly promoted worldwide by classical breeding and induced mutation techniques. The latter has enabled development of numerous favored varieties. Classical mutation combined with tissue culture have led to realize complete package of mutation techniques to develop new varieties through genetic manipulations for vegetative propagated ornamentals. A wide range of advanced techniques (TILLING, DNA-based techniques, etc.) have been standardized, which claim to be effective promoting the existing characteristics prospecting more beneficial novel types. Each method, along with its supporting parameters, contributes logistically to the development of a new variety. For an improvement program, one should have up-to-date knowledge about technical details and their merits and demerits. Classical mutation technique is now well organized for almost all ornamental plants, but EMS-TILLING, EcoTILLING, NGS-TILLING promises added value. Whereas, gene editing opens new avenues, but its applications for ornamentals plants is still in its infancy. Breeders should be conscious about the pros and cons of differing perspective and should consciously select the plan which is logistically appropriate, along with budget, for achieving the target under the current situation of variety advancement. An attempt has been made in this review to evaluate the usefulness of all major techniques to substantiate mutation development in ornamentals. This article aligns with SDG 2 (Zero Hunger) of the UN Agenda for Sustainable Development.