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<p>Naturally occurring allelic variation was mined for genes responsible for controlling parthenocarpy in cucumber. During this investigation, two genes were selected; <i>CsARF4</i> (Auxin Response Factor) and <i>CsEIN1</i> (Ethylene insensitive) which directly/indirectly regulated parthenocarpy via regulation of plant hormone metabolism. Ten cucumber genotypes showing variation in parthenocarpic potential were used. Based on the Sanger sequencing data, SNPs were identified in the genes. On the basis of SNP data, two Kompetitive allele-specific PCR (KASP) markers were designed. Out of the two genes, marker for <i>CsARF4</i> gene showed heterozygous results. Based on the parthenocarpic data, the cucumber genotypes were clustered into two distinct groups showing low and high parthenocarpic behaviour. Thus, the KASP marker for gene <i>CsEIN1</i> could be used for validation of parthenocarpy in cucumber genotypes.</p>

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Allele mining for the candidate genes regulating parthenocarpy in cucumber (Cucumis sativus L.)

  • 期刊论文

  • 发表日期：

    2025年11月3日

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  • Harleen Kaur,
  • Pooja Manchanda,
  • Rajinder Kumar Dhall,
  • Gurwinder Kaur

摘要

Naturally occurring allelic variation was mined for genes responsible for controlling parthenocarpy in cucumber. During this investigation, two genes were selected; CsARF4 (Auxin Response Factor) and CsEIN1 (Ethylene insensitive) which directly/indirectly regulated parthenocarpy via regulation of plant hormone metabolism. Ten cucumber genotypes showing variation in parthenocarpic potential were used. Based on the Sanger sequencing data, SNPs were identified in the genes. On the basis of SNP data, two Kompetitive allele-specific PCR (KASP) markers were designed. Out of the two genes, marker for CsARF4 gene showed heterozygous results. Based on the parthenocarpic data, the cucumber genotypes were clustered into two distinct groups showing low and high parthenocarpic behaviour. Thus, the KASP marker for gene CsEIN1 could be used for validation of parthenocarpy in cucumber genotypes.