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<p>The genetic fidelity of mitochondrial (mt), chloroplast (cp) and nuclear genomes in enhanced axillary bud cultures of <i>in vitro</i> micropropagated plants of the five (I to V) elite clones of <i>Tectona grandis</i> raised from the adult elite selected mother plants was assessed using restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) DNA markers. Somaclonal variation was detected in the nuclear genomes of all the clones, and in mt genomes of clones II and V. RAPD and ISSR DNA markers detected subtle polymorphism in some micropropagated plants across all the clones. Additionally, novel changes in mt genome organization were detected in clones II and V. No polymorphism was detected in chloroplast genome. This is the first comprehensive report on the assessment of genetic integrity in the <i>in vitro</i> raised micropropagated plants of highly valued hard wood teak tree using DNA fingerprinting. The present results demonstrate that the analyzed enhanced axillary branching protocol of five elite teak clones needs optimization for clonal propagation and to harness the superior quality traits of the mother plants in the regenerants.</p>

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Genetic fidelity analysis of tissue culture-derived teak (Tectona grandis) elite clones

  • 期刊论文

  • 发表日期：

    2025年7月4日

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  • Soumen Nandy,
  • Vachaspati Mishra,
  • Vijay Rani Rajpal,
  • Apekshita Singh,
  • Rachayya Devarumath,
  • Dhananjay Raturi,
  • Riddhima Singh,
  • Manju Chaudhary,
  • Soom Nath Raina

摘要

The genetic fidelity of mitochondrial (mt), chloroplast (cp) and nuclear genomes in enhanced axillary bud cultures of in vitro micropropagated plants of the five (I to V) elite clones of Tectona grandis raised from the adult elite selected mother plants was assessed using restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) DNA markers. Somaclonal variation was detected in the nuclear genomes of all the clones, and in mt genomes of clones II and V. RAPD and ISSR DNA markers detected subtle polymorphism in some micropropagated plants across all the clones. Additionally, novel changes in mt genome organization were detected in clones II and V. No polymorphism was detected in chloroplast genome. This is the first comprehensive report on the assessment of genetic integrity in the in vitro raised micropropagated plants of highly valued hard wood teak tree using DNA fingerprinting. The present results demonstrate that the analyzed enhanced axillary branching protocol of five elite teak clones needs optimization for clonal propagation and to harness the superior quality traits of the mother plants in the regenerants.