Scholarlink

<p>Vitamin C and Vitamin E, both are important for human health as antioxidants and prevent several human diseases. <i>Capsicum</i> is a good source of Vitamin C and E. Only few genes involved in Vitamin C biosynthesis were reported earlier in <i>Capsicum annuum</i>, and no report of identification of genes involved in Vitamin E biosynthesis in <i>Capsicum</i> is available till date. Therefore, in this study, we estimate Vitamin C and E content and comprehensively identify Vitamin C and E biosynthesis genes in three <i>Capsicum</i> species. Our analysis of Vitamin C and E contents in 30 <i>Capsicum</i> genotypes belonging to <i>C. annuum, C. chinense,</i> and <i>C. frutescens</i> showed the highest Vitamin C content of 35.87&#xa0;mg/g, 34.38&#xa0;mg/g, and 41.76&#xa0;mg/g, respectively. Similarly, the highest Vitamin E content was 111.4&#xa0;mg/g, 108.7&#xa0;mg/g, and 112.4&#xa0;mg/g, respectively. Overall, Vitamin C content was found higher in the majority of <i>C. chinense</i> genotypes, while Vitamin E content was observed higher in the <i>C. annuum</i> genotypes. Further, a comprehensive identification of Vitamin C and Vitamin E genes identified a total of 110 (29 Vit C &amp; 81 Vit E), 129 (44 Vit C &amp; 85 Vit E), and 106 (36 Vit C &amp; 70 Vit E) genes in <i>C. annuum</i>, <i>C. baccatum</i>, and <i>C. chinense</i> genome, respectively. The transcriptome and qRT-PCR analysis in the three <i>Capsicum</i> species showed significant correlations between gene expression and Vitamin C and E content for the majority of genes. Furthermore, we developed 24 and 37 polymorphic SSR and InDel markers based on the genes and 2&#xa0;KB upstream region of the genes, respectively.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification and expression analysis of genes involved in biosynthesis of Vitamin C and E in Capsicum species

  • 期刊论文

  • 发表日期：

    2025年6月21日

查看全文
  • Meenakshi Dubey,
  • Khushbu Islam,
  • Abdul Rawoof,
  • Neha Tiwari,
  • Vandana Jaiswal,
  • Ajay Kumar,
  • Jai Gopal Sharma,
  • Nirala Ramchiary

摘要

Vitamin C and Vitamin E, both are important for human health as antioxidants and prevent several human diseases. Capsicum is a good source of Vitamin C and E. Only few genes involved in Vitamin C biosynthesis were reported earlier in Capsicum annuum, and no report of identification of genes involved in Vitamin E biosynthesis in Capsicum is available till date. Therefore, in this study, we estimate Vitamin C and E content and comprehensively identify Vitamin C and E biosynthesis genes in three Capsicum species. Our analysis of Vitamin C and E contents in 30 Capsicum genotypes belonging to C. annuum, C. chinense, and C. frutescens showed the highest Vitamin C content of 35.87 mg/g, 34.38 mg/g, and 41.76 mg/g, respectively. Similarly, the highest Vitamin E content was 111.4 mg/g, 108.7 mg/g, and 112.4 mg/g, respectively. Overall, Vitamin C content was found higher in the majority of C. chinense genotypes, while Vitamin E content was observed higher in the C. annuum genotypes. Further, a comprehensive identification of Vitamin C and Vitamin E genes identified a total of 110 (29 Vit C & 81 Vit E), 129 (44 Vit C & 85 Vit E), and 106 (36 Vit C & 70 Vit E) genes in C. annuum, C. baccatum, and C. chinense genome, respectively. The transcriptome and qRT-PCR analysis in the three Capsicum species showed significant correlations between gene expression and Vitamin C and E content for the majority of genes. Furthermore, we developed 24 and 37 polymorphic SSR and InDel markers based on the genes and 2 KB upstream region of the genes, respectively.