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<p>Chitosan of various molecular weights are known to have biocompatibility, angiogenic, antimicrobial, and in vitro anti-inflammatory effects. However, the intricacies of wound healing using hydrogel formulations in vivo require further investigation. In this study, high-molecular-weight chitosan (HMWC) was sequentially treated with immobilized chitosanase to synthesize active molecular chitosan (AMC). The antimicrobial activity and wound-healing effect of AMC-based hydrogels were evaluated. Two types of AMC-based hydrogel, namely AMC-based hydrogel-h (AMC-h) from the hydrolysis of HMWC and AMC-based hydrogel-w (AMC-w) from water-soluble chitosan, were investigated for efficacy against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and in vivo wound healing. Subsequently, 48 full-thickness wounds were created in the BALB/c nude mice. The wounds were treated with AMC-h, AMC-w, or phosphate-buffered saline as controls. Collagen synthesis and the production of VEGF and TGF-β were quantified using ELISA and immunohistochemistry. AMC-h treatment significantly reduced the unhealed wound areas and displayed potent antimicrobial activity. It also promoted higher collagen synthesis and more substantial wound contraction than AMC-w. Taken together, our results demonstrate that AMC-h has the potential to accelerate wound healing using hydrogel formulations by enhancing antimicrobial activity, collagen synthesis, and wound contraction.</p> Graphical abstract <p></p>

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Synthesis of active molecular chitosan with selective molecular weight and its potential to promote wound healing

  • 期刊论文

  • 发表日期：

    2025年5月7日

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  • Eung Take Lee,
  • Hyun Jyung Oh,
  • Yewon Choi,
  • Hong Seog Seo,
  • Yong Hyun Lee,
  • Jae Kweon Park,
  • Sik Namgoong

摘要

Chitosan of various molecular weights are known to have biocompatibility, angiogenic, antimicrobial, and in vitro anti-inflammatory effects. However, the intricacies of wound healing using hydrogel formulations in vivo require further investigation. In this study, high-molecular-weight chitosan (HMWC) was sequentially treated with immobilized chitosanase to synthesize active molecular chitosan (AMC). The antimicrobial activity and wound-healing effect of AMC-based hydrogels were evaluated. Two types of AMC-based hydrogel, namely AMC-based hydrogel-h (AMC-h) from the hydrolysis of HMWC and AMC-based hydrogel-w (AMC-w) from water-soluble chitosan, were investigated for efficacy against methicillin-resistant Staphylococcus aureus (MRSA) and in vivo wound healing. Subsequently, 48 full-thickness wounds were created in the BALB/c nude mice. The wounds were treated with AMC-h, AMC-w, or phosphate-buffered saline as controls. Collagen synthesis and the production of VEGF and TGF-β were quantified using ELISA and immunohistochemistry. AMC-h treatment significantly reduced the unhealed wound areas and displayed potent antimicrobial activity. It also promoted higher collagen synthesis and more substantial wound contraction than AMC-w. Taken together, our results demonstrate that AMC-h has the potential to accelerate wound healing using hydrogel formulations by enhancing antimicrobial activity, collagen synthesis, and wound contraction.

Graphical abstract