Introduction <p>Vaginal vault closure is a crucial step in minimally invasive hysterectomy, and the choice of suture material can influence operative efficiency and postoperative outcomes. Barbed sutures, with their knotless self-anchoring design, may overcome technical limitations of conventional polyglactin sutures. This study compares barbed and non-barbed sutures for vault closure with respect to operative time, postoperative complications, and suture absorption.</p> Materials and Methods <p>This retrospective cohort study included 204 women who underwent laparoscopic or robotic hysterectomy for benign gynecological indications between July’2016 – April’2024. Vaginal vault closure was performed using either vicryl-1 (non-barbed; n=100) or V-Loc 1-0 (barbed; n=104). Data on demographic variables, comorbidities, suturing time, postoperative complications, and suture absorption were collected. Follow-up was at 48 hours, 1 week, and at 6, 10, and 12 weeks. Statistical analysis used Chi-square and Student’s t-tests; p&lt;0.05 was considered significant.</p> Results <p>Baseline demographic and clinical characteristics were comparable between groups. Mean suturing time was significantly shorter with barbed sutures (10.28 ± 1.94 vs 12.69 ± 3.31 minutes; p &lt; 0.0001). Secondary hemorrhage (2.88% vs 12%; p = 0.0126) and vault granuloma (1.92% vs 10%; p = 0.0142) were significantly lower with barbed sutures. One case of vaginal cuff dehiscence occurred in the non-barbed group. Suture absorption was delayed with barbed sutures, with only 43.26% absorbed by 12 weeks compared to complete absorption of vicryl by 10 weeks.</p> Conclusion <p>Barbed sutures offer improved operative efficiency and lower postoperative complication rates for vaginal vault closure in minimally invasive hysterectomy. However, delayed suture absorption warrants appropriate patient counseling.</p>