<p>Owing to the global prohibition of cosmetics tested on animals, there has been a substantial surge in the demand for functional skin models. Using a silk fibroin scaffold approved for human implantation, we successfully fabricated a contraction-free full-thickness human skin equivalent by culturing fibroblasts on the scaffold without using animal collagen, addressing the contraction issues associated with 3D scaffolds containing animal collagen. Optimization of the pore size distribution and porosity of the scaffold was achieved based on the NaCl particle distribution. A comparative analysis of a skin-on-a-chip model was conducted, considering the histology, barrier layer protein expression, and other factors, with or without the presence of vascular endothelial cells. We observed the formation of stratum corneum layers constituting the skin barrier regardless of the scaffold thickness when vascular endothelial cells were employed. Consequently, contraction-free full-thickness skin equivalents made exclusively from materials approved for human implantation can be applied in various fields, including skin wound healing, assessment of therapeutic performance for skin diseases, evaluation of the side effects of therapeutic drugs, and alternative cosmetic animal testing.</p>