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<p>DNA-templated fluorescent copper nanoclusters (CuNCs) are cost-effective alternatives to gold and silver nanoclusters for diagnostic purposes. However, they are vulnerable to oxidation; thus, their fluorescence only lasts for a short period in solution, limiting their widespread application. In this study, we used glycerol as a stabilizing agent to enhance the fluorescence stability of CuNCs and successfully constructed a fluorescent lateral flow assay (LFA) system. The presence of glycerol enabled the maintenance of high fluorescence signals for over 2&#xa0;h, which was significantly improved compared to the CuNCs without glycerol, whose fluorescence completely disappeared within 5&#xa0;min. Using an LFA system with glycerol-stabilized CuNCs as detection labels, a fluorescent detection system for target nucleic acids that showed good sensitivity and selectivity was developed. In addition, the method was expanded to analyze two target nucleic acids, confirming the feasibility of multiplex detection. These findings suggest that glycerol is an effective stabilizer of fluorescent CuNCs and may contribute to developing more reliable and accessible fluorescence-based diagnostic systems.</p>

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Lateral Flow Assay System Using Glycerol-Stabilized Fluorescent Copper Nanoclusters as Detection Labels

  • 期刊论文

  • 发表日期：

    2025年9月4日

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  • Jimin Jeon,
  • Min Cheol Cho,
  • Subin Kim,
  • Seok Hyun Kim,
  • Seung Hyeon Reo,
  • Seokjoon Kim,
  • Ki Soo Park

摘要

DNA-templated fluorescent copper nanoclusters (CuNCs) are cost-effective alternatives to gold and silver nanoclusters for diagnostic purposes. However, they are vulnerable to oxidation; thus, their fluorescence only lasts for a short period in solution, limiting their widespread application. In this study, we used glycerol as a stabilizing agent to enhance the fluorescence stability of CuNCs and successfully constructed a fluorescent lateral flow assay (LFA) system. The presence of glycerol enabled the maintenance of high fluorescence signals for over 2 h, which was significantly improved compared to the CuNCs without glycerol, whose fluorescence completely disappeared within 5 min. Using an LFA system with glycerol-stabilized CuNCs as detection labels, a fluorescent detection system for target nucleic acids that showed good sensitivity and selectivity was developed. In addition, the method was expanded to analyze two target nucleic acids, confirming the feasibility of multiplex detection. These findings suggest that glycerol is an effective stabilizer of fluorescent CuNCs and may contribute to developing more reliable and accessible fluorescence-based diagnostic systems.