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<p>Melanoma, the most lethal form of skin cancer, is closely associated with tyrosinase activity, making it a critical biomarker for early diagnosis. In this study, we developed a dual-mode biosensing platform integrating multicolorimetric and ratiometric fluorometric detection for ultra-sensitive tyrosinase quantification. This platform leverages localized surface plasmon resonance (LSPR) modulation of gold nanobipyramids (AuNBPs) alongside a fluorescence resonance energy transfer (FRET)-based mechanism using upconversion nanoparticles (UCNPs). The tunable LSPR of AuNBPs provided a bidirectional ratiometric multicolor emission improving sensitivity and selectivity. Tyrosinase-mediated oxidation of catechol to quinone inhibits silver (Ag) deposition on AuNBPs, inducing a distinct concentration-dependent color shift for precise visual quantification. This dual-modality assay achieves an exceptional limit of detection (LOD) of 4.13 × 10<sup>–5</sup> and 2.71 × 10<sup>–5</sup>&#xa0;U/mL for colorimetric and fluorometric sensors, respectively, demonstrating high selectivity, robustness in complex biological samples. Additionally, the assay proved effective in screening tyrosinase inhibitors, using kojic acid as a model, and achieved an IC<sub>50</sub> value of 61.45&#xa0;µM, validating its inhibition efficiency.</p>

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Integrated Colorimetric and Fluorescent Assay Using Gold Nanobipyramids and UCNPs for Tyrosinase-based Melanoma Diagnostics

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月7日

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  • Mohamed R. Elmasry,
  • Samy M. Shaban,
  • Murad M. A. Abualrejal,
  • Eslam Hafez,
  • Ahmed Y. Elbalaawy,
  • Siqi Sun,
  • Dong-Hwan Kim

摘要

Melanoma, the most lethal form of skin cancer, is closely associated with tyrosinase activity, making it a critical biomarker for early diagnosis. In this study, we developed a dual-mode biosensing platform integrating multicolorimetric and ratiometric fluorometric detection for ultra-sensitive tyrosinase quantification. This platform leverages localized surface plasmon resonance (LSPR) modulation of gold nanobipyramids (AuNBPs) alongside a fluorescence resonance energy transfer (FRET)-based mechanism using upconversion nanoparticles (UCNPs). The tunable LSPR of AuNBPs provided a bidirectional ratiometric multicolor emission improving sensitivity and selectivity. Tyrosinase-mediated oxidation of catechol to quinone inhibits silver (Ag) deposition on AuNBPs, inducing a distinct concentration-dependent color shift for precise visual quantification. This dual-modality assay achieves an exceptional limit of detection (LOD) of 4.13 × 10–5 and 2.71 × 10–5 U/mL for colorimetric and fluorometric sensors, respectively, demonstrating high selectivity, robustness in complex biological samples. Additionally, the assay proved effective in screening tyrosinase inhibitors, using kojic acid as a model, and achieved an IC50 value of 61.45 µM, validating its inhibition efficiency.