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<p>DNA possesses unique abilities such as informational self-assembly and digital data storage with high density. Here, we demonstrate the deep-UV photolithography for DNA data storage with diverse functionalities such as content addressing, identification, and long-term storage. Using fluorescent DNA hybridization for DNA deep-UV photolithography, single-strand breaks (SSB) showed clearer patterns. DNA deep-UV photolithography with fluorescent DNA hybridization created a quick response (QR) pattern, which has the functionality of content addressing. The fluorescent DNA QR patterns, which were created by SSB, were linked to different informational websites, using smartphone QR image applications. Furthermore, a sentence was encoded in a 29 × 29-pixel QR pattern with a Reed-Solomon error correction code, and DNA sequences were synthesized with fluorescence. The fluorescent DNA sequences were selectively hybridized on the QR pattern substrate, and the patterns were synthesized using fluorescent dNTP. The fluorescent QR DNA was synthesized using primers and patterned on the substrate for DNA data storage. Even though we provided limited demonstrations such as data capacity and content image for DNA data storage, the use of DNA photolithography to create and store 2D pattern information could be extended to diverse applications for random access storage.</p>

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Polymerase Elongation onto Patterned DNA for Random Accessed DNA Data Storage

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Taeseok Kang,
  • Doyeon Lim,
  • Wonjin Lee,
  • Youngjun Song

摘要

DNA possesses unique abilities such as informational self-assembly and digital data storage with high density. Here, we demonstrate the deep-UV photolithography for DNA data storage with diverse functionalities such as content addressing, identification, and long-term storage. Using fluorescent DNA hybridization for DNA deep-UV photolithography, single-strand breaks (SSB) showed clearer patterns. DNA deep-UV photolithography with fluorescent DNA hybridization created a quick response (QR) pattern, which has the functionality of content addressing. The fluorescent DNA QR patterns, which were created by SSB, were linked to different informational websites, using smartphone QR image applications. Furthermore, a sentence was encoded in a 29 × 29-pixel QR pattern with a Reed-Solomon error correction code, and DNA sequences were synthesized with fluorescence. The fluorescent DNA sequences were selectively hybridized on the QR pattern substrate, and the patterns were synthesized using fluorescent dNTP. The fluorescent QR DNA was synthesized using primers and patterned on the substrate for DNA data storage. Even though we provided limited demonstrations such as data capacity and content image for DNA data storage, the use of DNA photolithography to create and store 2D pattern information could be extended to diverse applications for random access storage.