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<p>Oxygen-terminated silicon carbide (SiC) particle-coated electrodes are presented and applied in highly sensitive electrochemical immunoassays. The SiC particles used for electrode coating were characterized and compared in terms of their crystal structures and chemical bonding states through X-ray diffraction, Raman&#xa0;spectroscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The electrochemical properties of oxygen-terminated SiC particle-coated electrodes, including double layer capacitance and ideal polarizable potential range (electrochemical window), were analyzed and compared with those of bare carbon, Nafion-coated, and bare SiC particle-coated electrodes. Cyclic voltametric analysis was conducted using potassium ferricyanide, ferrocene, and 3,3′,5,5′-tetramethylbenzidine as model redox couples to evaluate the properties of the electrodes. Finally, the oxygen-terminated SiC particle-coated electrode was applied in commercial immunoassay kits for the medical diagnosis of the human immunodeficiency virus and human hepatitis-C virus. Amperometric measurements using the oxygen-terminated SiC particle-coated electrode demonstrated lower detection limits compared to those of conventional optical density measurements.</p>

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Oxygen-Terminated Silicon Carbide (SiC) Particle-Coated Electrodes for Highly Sensitive Electrochemical Immunoassays

  • 期刊论文

  • 发表日期：

    2025年2月27日

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  • Eunyoung Kim,
  • Seong-Min Jeong,
  • Ga-Yeon Lee

摘要

Oxygen-terminated silicon carbide (SiC) particle-coated electrodes are presented and applied in highly sensitive electrochemical immunoassays. The SiC particles used for electrode coating were characterized and compared in terms of their crystal structures and chemical bonding states through X-ray diffraction, Raman spectroscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The electrochemical properties of oxygen-terminated SiC particle-coated electrodes, including double layer capacitance and ideal polarizable potential range (electrochemical window), were analyzed and compared with those of bare carbon, Nafion-coated, and bare SiC particle-coated electrodes. Cyclic voltametric analysis was conducted using potassium ferricyanide, ferrocene, and 3,3′,5,5′-tetramethylbenzidine as model redox couples to evaluate the properties of the electrodes. Finally, the oxygen-terminated SiC particle-coated electrode was applied in commercial immunoassay kits for the medical diagnosis of the human immunodeficiency virus and human hepatitis-C virus. Amperometric measurements using the oxygen-terminated SiC particle-coated electrode demonstrated lower detection limits compared to those of conventional optical density measurements.