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<p>Stress influences plant growth and development, although beneficial in adoptive mechanisms but usually negatively affect production. The molecular response of plants to drought stress involves complex regulatory mechanisms which mainly involve changes in the transcriptional activity of stress-related genes. Transgenic plants provide critical insights to investigate the function of these genes. AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN (<i>AHL15</i>)is one of these genes which modulate plant morphology and developmental processes in <i>Arabidopsis</i> and tobacco. In this study, we evaluated the drought stress resistance of inducible <i>AHL15GR</i> transgenic <i>N. tabacum</i> by withholding water for 25 days. Our results show that shoot length and leaf size were reduced in the <i>AHL15GR</i> transgenic plants both before and after drought stress in the case of DEX activation compared to the wild type and Non-DEX treated <i>AHL15GR</i> transgenic tobacco plants (control). The chlorophyll content was significantly higher in the transgenic plants (13&#xa0;mg/g and 17&#xa0;mg/g) than in the wild-type plants (7&#xa0;mg/g and 9&#xa0;mg/g) during and after drought stress, respectively. Similarly, a significant increase in proline content was observed in DEX-treated <i>AHL15GR</i> transgenic tobacco plants (3.3µmoL/g and 2.7µmoL/g) as compared to wild-type plants (2.1µmoL/g and 1.9µmoL/g).The highest relative water content (RWC) was observed in <i>AHL15GR </i>transgenic plants before drought stress. Before drought stress, the RWC in DEX-induced <i>AHL15GR</i> transgenic tobacco plants was 60% compared to wild-type plants (35%), while after stress, an RWC of 50% was observed in DEX-activated <i>AHL15GR</i> transgenic plants compared to wild-type plants (40%). The increase in proline content, maintenance of chlorophyll and percentage RWC confirm that the <i>AHL15</i> gene induces drought resistance in the <i>AHL15</i>GR transgenic tobacco plants. These findings highlight a gene-driven pathway to build climate-resilient crops, which is essential for ensuring food security and sustainability in water-scarce regions.</p>

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Characterization of drought tolerance traits in Ahl15gr inducible transgenic Nicotiana tabacum L. (Solanaceae)

  • 期刊论文

  • 发表日期：

    2026年7月25日

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  • Zindagi Shakeel,
  • Majid Khan,
  • Israr Ud Din,
  • Muhammad Nauman Khan,
  • Majid Iqbal,
  • Alevcan Kaplan,
  • M. Ajmal Ali,
  • Mohamed S. Elshikh

摘要

Stress influences plant growth and development, although beneficial in adoptive mechanisms but usually negatively affect production. The molecular response of plants to drought stress involves complex regulatory mechanisms which mainly involve changes in the transcriptional activity of stress-related genes. Transgenic plants provide critical insights to investigate the function of these genes. AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN (AHL15)is one of these genes which modulate plant morphology and developmental processes in Arabidopsis and tobacco. In this study, we evaluated the drought stress resistance of inducible AHL15GR transgenic N. tabacum by withholding water for 25 days. Our results show that shoot length and leaf size were reduced in the AHL15GR transgenic plants both before and after drought stress in the case of DEX activation compared to the wild type and Non-DEX treated AHL15GR transgenic tobacco plants (control). The chlorophyll content was significantly higher in the transgenic plants (13 mg/g and 17 mg/g) than in the wild-type plants (7 mg/g and 9 mg/g) during and after drought stress, respectively. Similarly, a significant increase in proline content was observed in DEX-treated AHL15GR transgenic tobacco plants (3.3µmoL/g and 2.7µmoL/g) as compared to wild-type plants (2.1µmoL/g and 1.9µmoL/g).The highest relative water content (RWC) was observed in AHL15GR transgenic plants before drought stress. Before drought stress, the RWC in DEX-induced AHL15GR transgenic tobacco plants was 60% compared to wild-type plants (35%), while after stress, an RWC of 50% was observed in DEX-activated AHL15GR transgenic plants compared to wild-type plants (40%). The increase in proline content, maintenance of chlorophyll and percentage RWC confirm that the AHL15 gene induces drought resistance in the AHL15GR transgenic tobacco plants. These findings highlight a gene-driven pathway to build climate-resilient crops, which is essential for ensuring food security and sustainability in water-scarce regions.