Scholarlink

<p>Bacterial and fungal communities associated with mango pulp and seed kernels from eight Indian mango varieties were profiled using 16&#xa0;S rRNA and ITS amplicon sequencing. Bacterial diversity was consistently higher in seed kernels (647 ± 238 OTUs) than in pulp tissues (196 ± 112 OTUs). Seed kernel–associated bacterial communities were dominated by Firmicutes (35.8–44.0%) and Bacteroidota (16.8–35.8%) and showed high compositional consistency across varieties, with core genera including <i>Prevotella</i>, <i>Ruminiclostridium</i>, and <i>Lachnoclostridium</i>. In contrast, pulp-associated bacterial communities were enriched in Proteobacteria (6.5–88.5%) and Actinobacteria (4.4–34.6%) and exhibited pronounced inter-varietal variability, particularly in the relative abundance of Bacteroidota (0.8–53.8%). Fungal communities displayed lower richness (14–72 OTUs) and higher variability, with <i>Candida kruisii</i> (15–67%) and <i>Hanseniaspora uvarum</i> (up to 86%) as dominant taxa. Non-metric multidimensional scaling and hierarchical clustering revealed clear tissue-driven segregation of bacterial communities, whereas fungal assemblages showed weaker tissue-associated structuring. Seed kernels harbored approximately 3.3-fold more unique bacterial OTUs than pulp tissues, with the Amrapali seedkernel exhibiting the highest richness (789 OTUs). Across varieties, 82% of kernel-associated bacterial OTUs were shared, compared with 31% in pulp, indicating a conserved kernel microbiome and a more variable, cultivar-specific pulp microbiome. These results highlight strong tissue-level compartmentalization of mango-associated bacterial communities across cultivars.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metagenomic profiling of endophytic microbiomes associated with fruit pulp and seed kernels of different mango varieties reveals conservation of bacterial communities in seed kernels

  • 期刊论文

  • 发表日期：

    2026年5月25日

查看全文
  • Basavaprabhu L. Patil,
  • C. Shanmugaraj,
  • M. Madhusudan

摘要

Bacterial and fungal communities associated with mango pulp and seed kernels from eight Indian mango varieties were profiled using 16 S rRNA and ITS amplicon sequencing. Bacterial diversity was consistently higher in seed kernels (647 ± 238 OTUs) than in pulp tissues (196 ± 112 OTUs). Seed kernel–associated bacterial communities were dominated by Firmicutes (35.8–44.0%) and Bacteroidota (16.8–35.8%) and showed high compositional consistency across varieties, with core genera including Prevotella, Ruminiclostridium, and Lachnoclostridium. In contrast, pulp-associated bacterial communities were enriched in Proteobacteria (6.5–88.5%) and Actinobacteria (4.4–34.6%) and exhibited pronounced inter-varietal variability, particularly in the relative abundance of Bacteroidota (0.8–53.8%). Fungal communities displayed lower richness (14–72 OTUs) and higher variability, with Candida kruisii (15–67%) and Hanseniaspora uvarum (up to 86%) as dominant taxa. Non-metric multidimensional scaling and hierarchical clustering revealed clear tissue-driven segregation of bacterial communities, whereas fungal assemblages showed weaker tissue-associated structuring. Seed kernels harbored approximately 3.3-fold more unique bacterial OTUs than pulp tissues, with the Amrapali seedkernel exhibiting the highest richness (789 OTUs). Across varieties, 82% of kernel-associated bacterial OTUs were shared, compared with 31% in pulp, indicating a conserved kernel microbiome and a more variable, cultivar-specific pulp microbiome. These results highlight strong tissue-level compartmentalization of mango-associated bacterial communities across cultivars.