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<p>The present study reports the isolation and preliminary characterization of an endophytic fungal strain (FA-56) obtained from healthy leaves of <i>Ficus auriculata</i>. The isolate was identified as <i>Alternaria</i> sp. based on morphological characteristics and ITS rDNA sequence similarity. The crude ethyl acetate extract exhibited measurable antibacterial activity against six clinically relevant Gram-positive and Gram-negative bacteria, with inhibition zones ranging from 18.00 ± 1.00 to 20.33 ± 0.58&#xa0;mm. The highest antibacterial activity was observed against Gram negative bacteria <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i> with zone of inhibition of 20.33 ± 0.58&#xa0;mm and 19.33 ± 0.58&#xa0;mm, respectively. The extract further demonstrated inhibitory effects, with minimum inhibitory concentration (MIC) values ranging from 26.7 to 53.3&#xa0;µg mL⁻¹. Gas chromatography–mass spectrometry (GC–MS) profiling revealed several compounds tentatively identified based on library matching, including a dominant peak tentatively assigned to 2-fluorobenzoic acid, heptadecyl ester. As fluorinated compounds are uncommon among fungal metabolites, this identification should be considered tentative and interpreted with caution. These findings suggest that endophytic fungi associated with wild edible plants may serve as potential sources of antimicrobial metabolites. However, further purification, structural confirmation, and biological evaluation are required to substantiate their therapeutic relevance.</p>

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Antibacterial potential and metabolite profiling of an endophytic Alternaria sp. FA-56 isolated from Ficus auriculata, a wild edible plant of the Indo-Burma belt

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • Jwngma Basumatary,
  • Kumananda Tayung

摘要

The present study reports the isolation and preliminary characterization of an endophytic fungal strain (FA-56) obtained from healthy leaves of Ficus auriculata. The isolate was identified as Alternaria sp. based on morphological characteristics and ITS rDNA sequence similarity. The crude ethyl acetate extract exhibited measurable antibacterial activity against six clinically relevant Gram-positive and Gram-negative bacteria, with inhibition zones ranging from 18.00 ± 1.00 to 20.33 ± 0.58 mm. The highest antibacterial activity was observed against Gram negative bacteria Pseudomonas aeruginosa and Klebsiella pneumoniae with zone of inhibition of 20.33 ± 0.58 mm and 19.33 ± 0.58 mm, respectively. The extract further demonstrated inhibitory effects, with minimum inhibitory concentration (MIC) values ranging from 26.7 to 53.3 µg mL⁻¹. Gas chromatography–mass spectrometry (GC–MS) profiling revealed several compounds tentatively identified based on library matching, including a dominant peak tentatively assigned to 2-fluorobenzoic acid, heptadecyl ester. As fluorinated compounds are uncommon among fungal metabolites, this identification should be considered tentative and interpreted with caution. These findings suggest that endophytic fungi associated with wild edible plants may serve as potential sources of antimicrobial metabolites. However, further purification, structural confirmation, and biological evaluation are required to substantiate their therapeutic relevance.