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<p>We aim to produce and characterize superfine bacterial cellulose (BC) obtained from cost-effective fermentation at 37&#xa0;°C in coconut water (CW) using table sugar instead of sucrose as a carbon source. The low-cost BC with antibacterial activities was produced from the coculture fermentation of <i>Komagataeibacter oboediens</i> RC30-15 with <i>Saccharomyces bayanus</i> at 37&#xa0;°C in CW0.5A3S0.5N containing 0.5% acetic acid, 3% table sugar or sucrose and 0.5% ammonium sulfate. Scanning electron microscopic (SEM) analysis of BC film indicated no significant differences in BC fibrils’ average diameter between BC produced from sucrose or sugar as a carbon source at either 30&#xa0;or&#xa0;37&#xa0;°C. Moreover, there are no significant differences in the XRD patterns, FTIR spectra, and thermograms of BC produced from sucrose and sugar as carbon sources. Evaluation of antibacterial activities against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i> indicated that BC produced from sugar exhibited a similar inhibitory effect to that produced from sucrose. Coculture fermentation of <i>K. oboediens</i> RC30-15 with <i>S. bayanus</i> in CW containing sugar not only reduces the medium cost from 1.12 USD/L to 0.20 USD/L (1 USD=32 baht) but also enhances the antibacterial activities of BC. Additionally, BC produced by coculture fermentation showed a higher inhibitory effect than that by mixed-culture fermentation of <i>K. oboediens</i> RC30-15, <i>S. bayanus</i>, and <i>Pediococcus pentosaceus</i> DMKU 14-7. Antibacterial activity of BC against a chloramphenicol resistant strain of <i>P. aeruginosa</i> is interesting and might be applied as a natural antimicrobial agent, providing an alternative to antibiotics in the future.</p>

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Production and characterization of low-cost superfine bacterial cellulose from coculture fermentation of Komagataeibacter oboediens RC30-15 with Saccharomyces bayanus and its antibacterial activities

  • 期刊论文

  • 发表日期：

    2026年3月16日

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  • Punyawee Lertsirirut,
  • Uraiwan Tippayasak,
  • Buapan Puangsin,
  • Gunjana Theeragool

摘要

We aim to produce and characterize superfine bacterial cellulose (BC) obtained from cost-effective fermentation at 37 °C in coconut water (CW) using table sugar instead of sucrose as a carbon source. The low-cost BC with antibacterial activities was produced from the coculture fermentation of Komagataeibacter oboediens RC30-15 with Saccharomyces bayanus at 37 °C in CW0.5A3S0.5N containing 0.5% acetic acid, 3% table sugar or sucrose and 0.5% ammonium sulfate. Scanning electron microscopic (SEM) analysis of BC film indicated no significant differences in BC fibrils’ average diameter between BC produced from sucrose or sugar as a carbon source at either 30 or 37 °C. Moreover, there are no significant differences in the XRD patterns, FTIR spectra, and thermograms of BC produced from sucrose and sugar as carbon sources. Evaluation of antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa indicated that BC produced from sugar exhibited a similar inhibitory effect to that produced from sucrose. Coculture fermentation of K. oboediens RC30-15 with S. bayanus in CW containing sugar not only reduces the medium cost from 1.12 USD/L to 0.20 USD/L (1 USD=32 baht) but also enhances the antibacterial activities of BC. Additionally, BC produced by coculture fermentation showed a higher inhibitory effect than that by mixed-culture fermentation of K. oboediens RC30-15, S. bayanus, and Pediococcus pentosaceus DMKU 14-7. Antibacterial activity of BC against a chloramphenicol resistant strain of P. aeruginosa is interesting and might be applied as a natural antimicrobial agent, providing an alternative to antibiotics in the future.