Scholarlink

<p>The present study elucidates the oxidative stress response mechanisms of two marine cyanobacterial species, <i>Phormidium sp</i>. JMC111 and <i>Microcoleus acutissimus</i>, when exposed to hydrogen peroxide (H₂O₂). Both species tolerated short-term H₂O₂ stress up to 60&#xa0;min, exhibiting significant biochemical and enzymatic adaptations. Statistical analysis revealed a steady decline in chlorophyll content and degradation efficiency over time, with <i>M. acutissimus</i> maintaining higher resilience compared to <i>Phormidium</i> sp. JMC111. Non-enzymatic antioxidants such as carotenoids, β-carotene, tocopherol, and astaxanthin remained elevated, suggesting a strong photoprotective and radical-scavenging defense, while ascorbic acid and phycobiliproteins declined under prolonged stress. Two-way ANOVA confirmed significant time- and species-dependent variations (p &lt; 0.05) in antioxidant responses. Enzymatic assays on native PAGE revealed distinct isoform patterns of SOD, CAT, POD, and GPx, with the appearance of novel peroxidase and catalase isoforms under oxidative stress—reported here for the first time in marine cyanobacteria. These findings demonstrate that both cyanobacteria possess a dynamic and coordinated ROS defense network, with <i>M. acutissimus</i> showing superior antioxidative stability. The study identifies POD and CAT as reliable biochemical indicators of oxidative stress, establishing <i>Phormidium sp</i>. JMC111 as a potential biomonitor species for environmental oxidative assessment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Exploring the comprehensive ROS defense toolbox of selected marine cyanobacteria under hydrogen peroxide stress

  • 期刊论文

  • 发表日期：

    2026年1月5日

查看全文
  • J. Mohammed Hussain,
  • P. Muruganantham,
  • Khaleel Ahamed Abdul Kareem,
  • G. Jeevanantham,
  • R. Sabitha

摘要

The present study elucidates the oxidative stress response mechanisms of two marine cyanobacterial species, Phormidium sp. JMC111 and Microcoleus acutissimus, when exposed to hydrogen peroxide (H₂O₂). Both species tolerated short-term H₂O₂ stress up to 60 min, exhibiting significant biochemical and enzymatic adaptations. Statistical analysis revealed a steady decline in chlorophyll content and degradation efficiency over time, with M. acutissimus maintaining higher resilience compared to Phormidium sp. JMC111. Non-enzymatic antioxidants such as carotenoids, β-carotene, tocopherol, and astaxanthin remained elevated, suggesting a strong photoprotective and radical-scavenging defense, while ascorbic acid and phycobiliproteins declined under prolonged stress. Two-way ANOVA confirmed significant time- and species-dependent variations (p < 0.05) in antioxidant responses. Enzymatic assays on native PAGE revealed distinct isoform patterns of SOD, CAT, POD, and GPx, with the appearance of novel peroxidase and catalase isoforms under oxidative stress—reported here for the first time in marine cyanobacteria. These findings demonstrate that both cyanobacteria possess a dynamic and coordinated ROS defense network, with M. acutissimus showing superior antioxidative stability. The study identifies POD and CAT as reliable biochemical indicators of oxidative stress, establishing Phormidium sp. JMC111 as a potential biomonitor species for environmental oxidative assessment.