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<p>We identified and curated 14 full-length TRP channel genes in <i>Bemisia tabaci</i> Asia II-1, TRPA (TRPA1, TRPA5, Pain, Painless, Pyrexia, Waterwitch), TRPC (TRPL, TRPγ), TRPV (Nanchung, Inactive), TRPM, TRPN, TRPML, and TRPP (PKD1), from an initial 22 candidates using a 98% identity cutoff. Gene structures ranged from intron-less (Pain, Painless, Pyrexia, Waterwitch) to highly intron-rich (TRPN/TRPM: 24 exons/23 introns). Conserved motif/domain analyses recovered canonical Ion-transport and ankyrin-repeat signatures; TRPN uniquely showed eight predicted trans-membranes and the highest ankyrin count, while TRPM carried a SLOG motif, TRPML had ELD-TRPML domain, and PKD1 contained polycystin domain. Homology models (SWISS-MODEL; GMQE 0.45–0.88) displayed conserved S1-S4/S5-S6 architecture. DeepLoc predicted predominant plasma-membrane localization (probability &gt; 0.6 for most channels) with lysosomal/vacuolar co-localization for subsets (e.g., Pain, Pyrexia, Nanchung). Chromosomal mapping showed clustered distributions: Chr02 (Waterwitch, Pyrexia, Inactive), Chr03 (TRPL, Pain, Painless, TRPA1, Nanchung, TRPN), Chr04 (TRPA5, PKD1), Chr06 (TRPM, TRPγ), and solitary TRPML on Chr10. Phylogenetic analysis (IQ-TREE ML and NJ/RAxML) resolved seven subfamilies with strong support (bootstrap ≥ 85%), highlighting tight TRPA clustering and mechanotransductive TRPV pair (Nanchung/Inactive). qPCR across six tissues (calibrator: whole body) revealed antenna-enriched expression for Inactive (7.53 ± 0.44), Nanchung (5.88 ± 0.44), Waterwitch (5.77 ± 0.44), and TRPA1 (5.30 ± 0.44) (FDR &lt; 0.01), leg/wing showed elevation for Nanchung/Inactive/ TRPN, and abdominal upregulation of PKD1 (4.85 ± 0.06) and TRPM (4.28 ± 0.06). Developmentally (calibrator: adult), nymph and prepupa showed marked increase, e.g., Nanchung 14.90 ± 0.16, Inactive 12.40 ± 0.05, Waterwitch 11.84 ± 0.47, Pyrexia 8.25 ± 1.41 (FDR &lt; 0.01). ANOVA confirmed strong heterogeneity (stage-wise F &gt; 90, <i>p</i> &lt; 0.001) with Dunnett/FDR significance. These tissue and stage-biased profiles nominate Nanchung, Inactive, TRPA1, and TRPM/PKD1 as prioritized candidates for functional assays and stage-specific pest management.</p>

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Genomic landscape of TRP channels in Bemisia tabaci: identification and molecular characterization

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Amit Umesh Paschapur,
  • Sai Manoj Marella,
  • C. N. Rajarushi,
  • J. S. Pavan,
  • Sabtharishi Subramanian

摘要

We identified and curated 14 full-length TRP channel genes in Bemisia tabaci Asia II-1, TRPA (TRPA1, TRPA5, Pain, Painless, Pyrexia, Waterwitch), TRPC (TRPL, TRPγ), TRPV (Nanchung, Inactive), TRPM, TRPN, TRPML, and TRPP (PKD1), from an initial 22 candidates using a 98% identity cutoff. Gene structures ranged from intron-less (Pain, Painless, Pyrexia, Waterwitch) to highly intron-rich (TRPN/TRPM: 24 exons/23 introns). Conserved motif/domain analyses recovered canonical Ion-transport and ankyrin-repeat signatures; TRPN uniquely showed eight predicted trans-membranes and the highest ankyrin count, while TRPM carried a SLOG motif, TRPML had ELD-TRPML domain, and PKD1 contained polycystin domain. Homology models (SWISS-MODEL; GMQE 0.45–0.88) displayed conserved S1-S4/S5-S6 architecture. DeepLoc predicted predominant plasma-membrane localization (probability > 0.6 for most channels) with lysosomal/vacuolar co-localization for subsets (e.g., Pain, Pyrexia, Nanchung). Chromosomal mapping showed clustered distributions: Chr02 (Waterwitch, Pyrexia, Inactive), Chr03 (TRPL, Pain, Painless, TRPA1, Nanchung, TRPN), Chr04 (TRPA5, PKD1), Chr06 (TRPM, TRPγ), and solitary TRPML on Chr10. Phylogenetic analysis (IQ-TREE ML and NJ/RAxML) resolved seven subfamilies with strong support (bootstrap ≥ 85%), highlighting tight TRPA clustering and mechanotransductive TRPV pair (Nanchung/Inactive). qPCR across six tissues (calibrator: whole body) revealed antenna-enriched expression for Inactive (7.53 ± 0.44), Nanchung (5.88 ± 0.44), Waterwitch (5.77 ± 0.44), and TRPA1 (5.30 ± 0.44) (FDR < 0.01), leg/wing showed elevation for Nanchung/Inactive/ TRPN, and abdominal upregulation of PKD1 (4.85 ± 0.06) and TRPM (4.28 ± 0.06). Developmentally (calibrator: adult), nymph and prepupa showed marked increase, e.g., Nanchung 14.90 ± 0.16, Inactive 12.40 ± 0.05, Waterwitch 11.84 ± 0.47, Pyrexia 8.25 ± 1.41 (FDR < 0.01). ANOVA confirmed strong heterogeneity (stage-wise F > 90, p < 0.001) with Dunnett/FDR significance. These tissue and stage-biased profiles nominate Nanchung, Inactive, TRPA1, and TRPM/PKD1 as prioritized candidates for functional assays and stage-specific pest management.