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<p>Castor (<i>Ricinus communis</i> L.) in Euphorbiaceae family is an important tropical crop cultivated for seeds containing industrially valuable oil. With economic development, demand for castor beans and oil is rapidly increasing, yet production is severely affected by fungal diseases, particularly <i>Fusarium</i> and <i>Macrophomina</i>, due to limited advanced breeding methods. <i>F. oxysporum</i> f. sp. <i>ricini</i> -induced wilt in castor is a major pathogenic factor responsible for severe yield losses. Wilt resistance, a complex trait controlled by quantitative trait loci (QTLs), was investigated in this study by developing a linkage map and identifying novel QTLs in castor using F2:3 population. The was developed from the cross between two castor inbred lines, 48 − 1 (Jwala) and the wilt-susceptible genotype JI-35, and screened under pot conditions. Linkage map was developed using 71 SSR markers. A genetic map comprising 13 linkage groups, spanning a total of 1,028.7&#xa0;cM centimorgans (cM). Analysis of genotypic and phenotypic data from the mapping population, evaluated for wilt in pots, identified two QTLs on LG1 and 6 explaining 12.44–16.58% of phenotypic variation. PCR amplification using linked markers on LG 1 in resistant and susceptible F₄ families of the mapping population demonstrated that these markers effectively distinguish plants resistant or susceptible to wilt disease. These markers can be utilized for developing resistant varieties via backcross breeding and for screening germplasm at the seedling stage.</p>

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Development of linkage map and mapping of SSR markers linked to fusarium wilt resistance in F2:3 population of castor (Ricinus communis L.)

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Sushil Kumar,
  • Amar A. Sakure,
  • Hensi Kundaria,
  • Mukesh P. Patel,
  • Rumit Patel,
  • Pankaj Katba,
  • Kaplesh Patel

摘要

Castor (Ricinus communis L.) in Euphorbiaceae family is an important tropical crop cultivated for seeds containing industrially valuable oil. With economic development, demand for castor beans and oil is rapidly increasing, yet production is severely affected by fungal diseases, particularly Fusarium and Macrophomina, due to limited advanced breeding methods. F. oxysporum f. sp. ricini -induced wilt in castor is a major pathogenic factor responsible for severe yield losses. Wilt resistance, a complex trait controlled by quantitative trait loci (QTLs), was investigated in this study by developing a linkage map and identifying novel QTLs in castor using F2:3 population. The was developed from the cross between two castor inbred lines, 48 − 1 (Jwala) and the wilt-susceptible genotype JI-35, and screened under pot conditions. Linkage map was developed using 71 SSR markers. A genetic map comprising 13 linkage groups, spanning a total of 1,028.7 cM centimorgans (cM). Analysis of genotypic and phenotypic data from the mapping population, evaluated for wilt in pots, identified two QTLs on LG1 and 6 explaining 12.44–16.58% of phenotypic variation. PCR amplification using linked markers on LG 1 in resistant and susceptible F₄ families of the mapping population demonstrated that these markers effectively distinguish plants resistant or susceptible to wilt disease. These markers can be utilized for developing resistant varieties via backcross breeding and for screening germplasm at the seedling stage.