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<p>The depauperate and paleopolyploid family Myristicaceae face challenges for correct species identification. Majority rule consensus trees of morphomatrix of NER species reflected diagnostic group traits (<i>ca</i>. 41.70%)&#xa0;and variable traits (&#xa0;ca. 58.30%) respectively. Amplification and sequencing success rates with DNA barcode loci <i>rbcL</i>, <i>matK</i> and, <i>trnL-F</i> were &gt; 90% while 45% and 76% with <i>psbA-trnH</i>, respectively in <i>Horsfieldia</i> and <i>Knema</i>. 178 sequences of 8 species of the region were submitted to GenBank including <i>trnL-F</i> spacer mostly for the first time. The highest parsimony informative sites were exhibited by <i>trnL-F</i> and highest variable sites by <i>matK</i>. Mean inter-specific distances with <i>trnL-F</i>, <i>matK + trnL-F</i> in <i>Horsfieldia</i> and with <i>psbA-trnH</i>, <i>rbcL + trnL-F</i> in <i>Knema</i> were ≥ 2 times mean intraspecific distances. <i>trnL-F</i> in <i>Horsfieldia</i> and <i>rbcL + trnL-F</i> in <i>Knema</i>, and <i>trnL-F + psbA-trnH</i> in <i>Horsfieldia</i> and <i>Knema</i> showed barcode gaps. Identification success by ‘best match’ was 98% with <i>matK + trnL-F</i> and 96–97% with <i>trnL-F + psbA-trnH</i> in <i>Horsfieldia</i> and <i>Knema</i>. The Maximum Parsimony and Bayesian Inference (BI) trees with <i>trnL-F</i> and BI tree with <i>trnL-F + psbA-trnH</i> was monophyletic and resolved 100% and 60% species. Dynamic DNA QR codes generated with <i>Knema erratica</i>-<i>matK</i>, <i>Horsfieldia kingii</i>-<i>psbA-trnH</i>,<i> Endocomia macrocoma</i> subsp. <i>prainii-trnL-F</i>. The loci&#xa0;<i>trnL-F</i>,<i> trnL-F + psbA-trnH</i> were exhibited as candidate barcodes, additionally <i>matK + trnL-F</i> for <i>Horsfieldia</i> and <i>rbcL + trnL-F</i> for <i>Knema</i>. The DNA barcode library of NER Myristicaceae would aid in species identification, ecological variation and conservation biology.</p>

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DNA barcoding clubbed with morphomatrix of commonly distributed species of Myristicaceae from North East India

  • 期刊论文

  • 发表日期：

    2025年11月22日

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  • Rubi Barman,
  • Dipanwita Banik

摘要

The depauperate and paleopolyploid family Myristicaceae face challenges for correct species identification. Majority rule consensus trees of morphomatrix of NER species reflected diagnostic group traits (ca. 41.70%) and variable traits ( ca. 58.30%) respectively. Amplification and sequencing success rates with DNA barcode loci rbcL, matK and, trnL-F were > 90% while 45% and 76% with psbA-trnH, respectively in Horsfieldia and Knema. 178 sequences of 8 species of the region were submitted to GenBank including trnL-F spacer mostly for the first time. The highest parsimony informative sites were exhibited by trnL-F and highest variable sites by matK. Mean inter-specific distances with trnL-F, matK + trnL-F in Horsfieldia and with psbA-trnH, rbcL + trnL-F in Knema were ≥ 2 times mean intraspecific distances. trnL-F in Horsfieldia and rbcL + trnL-F in Knema, and trnL-F + psbA-trnH in Horsfieldia and Knema showed barcode gaps. Identification success by ‘best match’ was 98% with matK + trnL-F and 96–97% with trnL-F + psbA-trnH in Horsfieldia and Knema. The Maximum Parsimony and Bayesian Inference (BI) trees with trnL-F and BI tree with trnL-F + psbA-trnH was monophyletic and resolved 100% and 60% species. Dynamic DNA QR codes generated with Knema erratica-matK, Horsfieldia kingii-psbA-trnH, Endocomia macrocoma subsp. prainii-trnL-F. The loci trnL-F, trnL-F + psbA-trnH were exhibited as candidate barcodes, additionally matK + trnL-F for Horsfieldia and rbcL + trnL-F for Knema. The DNA barcode library of NER Myristicaceae would aid in species identification, ecological variation and conservation biology.