Scholarlink

<p>This study examined the physiological, biochemical, ultrastructural, and cytological effects of zinc (Zn), copper (Cu), zinc oxide (ZnO), copper oxide (CuO), and titanium dioxide (TiO<sub>2</sub>) nanoparticles (NPs) on <i>Digitalis ferruginea</i> subsp. <i>ferruginea</i> L. under in vitro conditions. Germination, stomatal conductance, photosynthetic rate, chlorophyll and carotenoid content, anatomical structures and cytotoxicity were analyzed. Germination percentages were not significantly affected by NP exposure (<i>p</i> = 0.800), although minor variations were measured between treatments. ZnO NPs at 20&#xa0;µg/mL increased photosynthetic rate (6.85 ± 1.01&#xa0;µmol&#xa0;m⁻<sup>2</sup>&#xa0;s⁻<sup>1</sup>), while CuO NPs at 5&#xa0;µg/mL induced strong inhibitory effects (0.79 ± 0.45&#xa0;µmol&#xa0;m⁻<sup>2</sup>&#xa0;s⁻<sup>1</sup>). CuO NPs at 7.5&#xa0;µg/mL significantly reduced chlorophyll a and b contents (<i>p</i> &lt; 0.001), and CuO at 2.5&#xa0;µg/mL caused a decrease in stomatal conductance (<i>p</i> = 0.009). TEM analysis showed that lower NP concentrations preserved cell wall and chloroplast integrity, while higher doses caused thylakoid disorganization, mitochondrial swelling, and vacuolization. Cytological observations presented that CuO, TiO<sub>2</sub>, and high ZnO concentrations triggered chromosomal abnormalities, such as anaphase bridges, spindle disturbances, and chromosome fragmentation. Root viability assays confirmed the membrane damage in CuO 2.5&#xa0;µg/mL (<i>p</i> &lt; 0.001), while ZnO 20&#xa0;µg/mL retained integrity. In conclusion, NP effects on <i>Digitalis ferruginea</i> subsp. <i>ferruginea</i> L. were dose- and type-dependent, with ZnO NPs exhibiting biostimulant properties at optimal concentrations and CuO NPs demonstrating cyto- and genotoxic effects. These findings point out the dual role of NPs as growth promoters and stress inducers, emphasizing the importance of NP exposure in plant biotechnological applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Application of nanoparticles as biostimulator for growth of Digitalis ferruginea subsp. ferruginea L. under in vitro conditions

  • 期刊论文

  • 发表日期：

    2025年10月31日

查看全文
  • Dipul Kumar Biswas,
  • Özge Kaya,
  • Ömer Can Ünüvar,
  • Mehmet Örgeç,
  • Sandeep Kumar Verma,
  • İlknur Dağ,
  • Mehmet Doğan,
  • Buhara Yücesan,
  • Muhammad Sameeullah,
  • Songül Gürel,
  • Ekrem Gürel

摘要

This study examined the physiological, biochemical, ultrastructural, and cytological effects of zinc (Zn), copper (Cu), zinc oxide (ZnO), copper oxide (CuO), and titanium dioxide (TiO2) nanoparticles (NPs) on Digitalis ferruginea subsp. ferruginea L. under in vitro conditions. Germination, stomatal conductance, photosynthetic rate, chlorophyll and carotenoid content, anatomical structures and cytotoxicity were analyzed. Germination percentages were not significantly affected by NP exposure (p = 0.800), although minor variations were measured between treatments. ZnO NPs at 20 µg/mL increased photosynthetic rate (6.85 ± 1.01 µmol m⁻2 s⁻1), while CuO NPs at 5 µg/mL induced strong inhibitory effects (0.79 ± 0.45 µmol m⁻2 s⁻1). CuO NPs at 7.5 µg/mL significantly reduced chlorophyll a and b contents (p < 0.001), and CuO at 2.5 µg/mL caused a decrease in stomatal conductance (p = 0.009). TEM analysis showed that lower NP concentrations preserved cell wall and chloroplast integrity, while higher doses caused thylakoid disorganization, mitochondrial swelling, and vacuolization. Cytological observations presented that CuO, TiO2, and high ZnO concentrations triggered chromosomal abnormalities, such as anaphase bridges, spindle disturbances, and chromosome fragmentation. Root viability assays confirmed the membrane damage in CuO 2.5 µg/mL (p < 0.001), while ZnO 20 µg/mL retained integrity. In conclusion, NP effects on Digitalis ferruginea subsp. ferruginea L. were dose- and type-dependent, with ZnO NPs exhibiting biostimulant properties at optimal concentrations and CuO NPs demonstrating cyto- and genotoxic effects. These findings point out the dual role of NPs as growth promoters and stress inducers, emphasizing the importance of NP exposure in plant biotechnological applications.