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<p>Nutrient optimization of <i>Lactobacillus plantarum</i> MTCC 1325 with cinnamon (40&#xa0;µg/mL) and pantothenic acid (4&#xa0;µg/mL) resulted in a chloroform extract of Media D that exhibited a strong activity profile relevant to the therapeutic approach to Alzheimer’s disease. Across 20–100&#xa0;µg/mL extracts were non-cytotoxic to undifferentiated PC12 cells. Chloroform extract of Media D showed potent radical scavenging [IC₅₀(superoxide) = 41.7 ± 2.1&#xa0;µg/mL, IC₅₀(NO) = 33.4 ± 1.1&#xa0;µg/mL], high total phenolics (68.6 ± 1.1&#xa0;mg GAE/g), and acetylcholinesterase inhibition [IC₅₀ = 33.2 ± 4.2&#xa0;µg/mL, galantamine (standard) = 22.5 ± 3.2&#xa0;µg/mL]. Pre-treatment with chloroform extract of Media D reduced LPS-induced cytotoxicity [IC₅₀ = 32.6 ± 3.6&#xa0;µg/mL] and lowered nitrite accumulation up to 1.69-fold versus LPS. GC–MS of the active fraction resolved six constituents, consistent with lipid and aromatic metabolites. Together, these results support a nutrient-first media design strategy, demonstrating that selective modulation of media composition under fixed incubation parameters can enrich <i>L. plantarum</i> culture-derived metabolites with antioxidants, anti-inflammatory, and AChE inhibitory activities related to the medical management of AD. This is a preliminary in vitro study using one strain and a single-cell model, Extract were crude and tested with modest replication, and library matched GC–MS identification. Mechanistic markers were not quantified, and in vivo validation is warranted.</p>

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Acetylcholinesterase inhibitory potency of Lactobacillus plantarum fermentation extracts, and antioxidant scavenging effects against LPS-induced nitric oxide production in PC12 cell lines

  • 期刊论文

  • 发表日期：

    2025年10月17日

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  • Shani Shine,
  • Vani Chandrapragasam

摘要

Nutrient optimization of Lactobacillus plantarum MTCC 1325 with cinnamon (40 µg/mL) and pantothenic acid (4 µg/mL) resulted in a chloroform extract of Media D that exhibited a strong activity profile relevant to the therapeutic approach to Alzheimer’s disease. Across 20–100 µg/mL extracts were non-cytotoxic to undifferentiated PC12 cells. Chloroform extract of Media D showed potent radical scavenging [IC₅₀(superoxide) = 41.7 ± 2.1 µg/mL, IC₅₀(NO) = 33.4 ± 1.1 µg/mL], high total phenolics (68.6 ± 1.1 mg GAE/g), and acetylcholinesterase inhibition [IC₅₀ = 33.2 ± 4.2 µg/mL, galantamine (standard) = 22.5 ± 3.2 µg/mL]. Pre-treatment with chloroform extract of Media D reduced LPS-induced cytotoxicity [IC₅₀ = 32.6 ± 3.6 µg/mL] and lowered nitrite accumulation up to 1.69-fold versus LPS. GC–MS of the active fraction resolved six constituents, consistent with lipid and aromatic metabolites. Together, these results support a nutrient-first media design strategy, demonstrating that selective modulation of media composition under fixed incubation parameters can enrich L. plantarum culture-derived metabolites with antioxidants, anti-inflammatory, and AChE inhibitory activities related to the medical management of AD. This is a preliminary in vitro study using one strain and a single-cell model, Extract were crude and tested with modest replication, and library matched GC–MS identification. Mechanistic markers were not quantified, and in vivo validation is warranted.