Scholarlink

<p>This study aimed to investigate the influence of different solvents on the extraction of bioactive compounds and the resulting biological activities of <i>P. rubra</i> leaves. Extracts were obtained via maceration using methanol, ethanol, ethyl acetate, and hexane, and evaluated for total phenolic content (TPC), antioxidant activity (via DPPH radical scavenging and β-carotene bleaching), antidiabetic activity (via α-glucosidase inhibition), and antibacterial activity against <i>Staphylococcus aureus</i>, <i>Salmonella typhimurium</i>, and <i>Escherichia coli</i>. The extracts were also analyzed using Fourier-transform infrared (FTIR) and liquid chromatography high-resolution mass spectrometry (LC-HRMS) to identify key functional groups and metabolite profiles. Methanol extract exhibited the highest TPC (482.22&#xa0;mg GAE/g) and α-glucosidase inhibitory activity (91.23%), while ethanol extract showed superior antioxidant activity. All extracts demonstrated antibacterial effects, with the methanol extract showing the strongest inhibition against <i>S. aureus</i> (10.71&#xa0;mm). LC-HRMS revealed that hexane extract contains mostly fatty acids and triterpenoids, while the primary compound, ursolic acid, was present in methanol, ethanol, and ethyl acetate at comparable quantities. Further chemometric analysis highlighted distinct metabolite profiles and identified pheophorbide A as a primary discriminant for extract classification. Our findings suggest that <i>P. rubra</i> leaves, particularly when extracted with polar solvents, are a promising source of natural antioxidants, antidiabetic agents, and antimicrobials for applications in pharmaceuticals, nutraceuticals, and functional foods. In addition, the solvent-dependent phytochemical profiles can provide direction for further optimization of solvent composition for targeted metabolite extraction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Solvent-dependent metabolite profile and in vitro biological activity of frangipani (Plumeria rubra) leaf extracts

  • 期刊论文

  • 发表日期：

    2025年9月29日

查看全文
  • Anastasia Wheni Indrianingsih,
  • Anjar Windarsih,
  • Salsa Meidika Asari,
  • Suci Indah Pratiwi,
  • Nadia Udanti Suwanda,
  • Eka Noviana,
  • Eka Rahayu,
  • Tri Wiyono

摘要

This study aimed to investigate the influence of different solvents on the extraction of bioactive compounds and the resulting biological activities of P. rubra leaves. Extracts were obtained via maceration using methanol, ethanol, ethyl acetate, and hexane, and evaluated for total phenolic content (TPC), antioxidant activity (via DPPH radical scavenging and β-carotene bleaching), antidiabetic activity (via α-glucosidase inhibition), and antibacterial activity against Staphylococcus aureus, Salmonella typhimurium, and Escherichia coli. The extracts were also analyzed using Fourier-transform infrared (FTIR) and liquid chromatography high-resolution mass spectrometry (LC-HRMS) to identify key functional groups and metabolite profiles. Methanol extract exhibited the highest TPC (482.22 mg GAE/g) and α-glucosidase inhibitory activity (91.23%), while ethanol extract showed superior antioxidant activity. All extracts demonstrated antibacterial effects, with the methanol extract showing the strongest inhibition against S. aureus (10.71 mm). LC-HRMS revealed that hexane extract contains mostly fatty acids and triterpenoids, while the primary compound, ursolic acid, was present in methanol, ethanol, and ethyl acetate at comparable quantities. Further chemometric analysis highlighted distinct metabolite profiles and identified pheophorbide A as a primary discriminant for extract classification. Our findings suggest that P. rubra leaves, particularly when extracted with polar solvents, are a promising source of natural antioxidants, antidiabetic agents, and antimicrobials for applications in pharmaceuticals, nutraceuticals, and functional foods. In addition, the solvent-dependent phytochemical profiles can provide direction for further optimization of solvent composition for targeted metabolite extraction.