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<p>Plant-based synthesis of nanoparticles offers a sustainable, biocompatible alternative to chemical methods. The present study reports the green synthesis and characterization of silver nanoparticles (AgNPs) using <i>Coleus aromaticus</i> aqueous extract (CA-AE) and evaluates their bio-efficacy. CA-AgNPs were characterized using UV–Vis spectroscopy, XRD, DLS, FTIR, and GC–MS. UV–Vis showed a surface plasmon resonance peak at 420&#xa0;nm; XRD confirmed a crystalline FCC structure with a 33.58&#xa0;nm average size. DLS analysis revealed particle sizes ranging from 10–120&#xa0;nm, mostly between 50–70&#xa0;nm. FTIR and GC–MS identified functional groups and ten phytocompounds, including methyl palmitate. Antioxidant assays showed CA-AgNPs had enhanced activity in DPPH (89.12%), H₂O₂ (74.66%), and FRAP (1.28 AAE) at 100&#xa0;µg/mL compared to CA-AE. Antibacterial studies indicated moderate inhibition against <i>Staphylococcus aureus</i> (ZOI: 5&#xa0;mm at 15&#xa0;µL) and limited activity against <i>Pseudomonas aeruginosa</i>. Larvicidal assays on <i>Aedes aegypti</i> revealed dose- and time-dependent mortality, with CA-AgNPs achieving 96% mortality at 100&#xa0;µg/mL after 72&#xa0;h (LC₅₀: 5.989&#xa0;µg/mL; LC₉₀: 49.564&#xa0;µg/mL), compared to CA-AE (88% mortality; LC₅₀: 9.634&#xa0;µg/mL; LC₉₀: 111.482&#xa0;µg/mL). Treated larvae showed gut blackening and deformities. In silico docking showed methyl palmitate bound to Sterol Carrier Protein-2 (−&#xa0;6.9&#xa0;kcal/mol) and Pheromone Binding Protein (−&#xa0;6.3&#xa0;kcal/mol). These results of the present study recommend the eco-friendly synthesis and biological potential of CA-AgNPs, particularly in vector control, highlighting their promise as sustainable alternatives to conventional chemical insecticides.</p>

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Fabrication of silver nanoparticles from Coleus aromaticus and its mosquito larvicidal property-in vitro and in silico

  • 期刊论文

  • 发表日期：

    2025年11月26日

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  • Paramasivam Deepak,
  • R. Nehaa,
  • Vanessa Harrison,
  • Devayani B. Pandey,
  • Rupesh Gupta,
  • Ajay Guru,
  • Siva Prasad Panda,
  • Uttam Prasad Panigrahy,
  • Vellingiri Manon Mani

摘要

Plant-based synthesis of nanoparticles offers a sustainable, biocompatible alternative to chemical methods. The present study reports the green synthesis and characterization of silver nanoparticles (AgNPs) using Coleus aromaticus aqueous extract (CA-AE) and evaluates their bio-efficacy. CA-AgNPs were characterized using UV–Vis spectroscopy, XRD, DLS, FTIR, and GC–MS. UV–Vis showed a surface plasmon resonance peak at 420 nm; XRD confirmed a crystalline FCC structure with a 33.58 nm average size. DLS analysis revealed particle sizes ranging from 10–120 nm, mostly between 50–70 nm. FTIR and GC–MS identified functional groups and ten phytocompounds, including methyl palmitate. Antioxidant assays showed CA-AgNPs had enhanced activity in DPPH (89.12%), H₂O₂ (74.66%), and FRAP (1.28 AAE) at 100 µg/mL compared to CA-AE. Antibacterial studies indicated moderate inhibition against Staphylococcus aureus (ZOI: 5 mm at 15 µL) and limited activity against Pseudomonas aeruginosa. Larvicidal assays on Aedes aegypti revealed dose- and time-dependent mortality, with CA-AgNPs achieving 96% mortality at 100 µg/mL after 72 h (LC₅₀: 5.989 µg/mL; LC₉₀: 49.564 µg/mL), compared to CA-AE (88% mortality; LC₅₀: 9.634 µg/mL; LC₉₀: 111.482 µg/mL). Treated larvae showed gut blackening and deformities. In silico docking showed methyl palmitate bound to Sterol Carrier Protein-2 (− 6.9 kcal/mol) and Pheromone Binding Protein (− 6.3 kcal/mol). These results of the present study recommend the eco-friendly synthesis and biological potential of CA-AgNPs, particularly in vector control, highlighting their promise as sustainable alternatives to conventional chemical insecticides.