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<p>In this study, we isolated, identified, and characterized the rhizobia associated with cowpea nodules from four agricultural soils in the northern (Piura) and central (Barranca) desert coast of Peru. The rhizobia were isolated and authenticated, then identified using Multi Locus Sequence Analysis (MLSA) of concatenated housekeeping genes. They were evaluated for their tolerance to abiotic factors and plant growth-promoting properties, as well as for their symbiotic efficiency. Sixty-five isolates were grouped into thirty-two BOX-PCR groups. Using the 16S ribosomal gene and an MLSA analysis of four housekeeping genes (<i>recA</i>, <i>glnII</i>, <i>gyrB</i>, and <i>dnaK</i>), the strains were shown to belong to eight genomic lineages within the <i>Bradyrhizobium</i> genus. Their similarity percentages (&gt; 97%) matched <i>B. diversitatis</i>, <i>B. yuanmingense</i>, <i>B. paxllaeri</i> and <i>B. retamae</i>, as well as four possible new species (&lt; 97%). The phylogeny of the symbiotic genes (&gt; 92.5%) indicated that the strains belong to the symbiovars americensis, cajani, retamae and a possible new symbiovar (91.5 – 91.8%). At least one strain demonstrated tolerance to 171&#xa0;mM NaCl and growth at 35&#xa0;°C, as well as the ability to solubilize phosphate and produce auxins or siderophores. Only 21.9% of the strains showed a symbiotic effectiveness greater than 80% compared to the uninoculated control. <i>B. diversitatis</i> and <i>B. yuanmingense</i> were identified as the most abundant rhizobia associated with cowpea in desert soils of the Peruvian coast. Strains exhibiting tolerance to abiotic factors, along with growth-promoting properties represent a valuable resource for the development of inoculants adapted to the desert conditions of the Peruvian coast.</p>

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Phylogenetic, phenotypic, and symbiotic characterization of Bradyrhizobium isolated from nodules of cowpea grown on the Peruvian desert coast

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Renzo A. Valdez-Nuñez,
  • Sayumi M. Melendre-Rodriguez,
  • Anderson R. Calixto-Garcia,
  • Celia C. Silvera-Pablo,
  • Antonio Castellano-Hinojosa,
  • Ernesto Ormeño-Orrillo,
  • Mariangela Hungria

摘要

In this study, we isolated, identified, and characterized the rhizobia associated with cowpea nodules from four agricultural soils in the northern (Piura) and central (Barranca) desert coast of Peru. The rhizobia were isolated and authenticated, then identified using Multi Locus Sequence Analysis (MLSA) of concatenated housekeeping genes. They were evaluated for their tolerance to abiotic factors and plant growth-promoting properties, as well as for their symbiotic efficiency. Sixty-five isolates were grouped into thirty-two BOX-PCR groups. Using the 16S ribosomal gene and an MLSA analysis of four housekeeping genes (recA, glnII, gyrB, and dnaK), the strains were shown to belong to eight genomic lineages within the Bradyrhizobium genus. Their similarity percentages (> 97%) matched B. diversitatis, B. yuanmingense, B. paxllaeri and B. retamae, as well as four possible new species (< 97%). The phylogeny of the symbiotic genes (> 92.5%) indicated that the strains belong to the symbiovars americensis, cajani, retamae and a possible new symbiovar (91.5 – 91.8%). At least one strain demonstrated tolerance to 171 mM NaCl and growth at 35 °C, as well as the ability to solubilize phosphate and produce auxins or siderophores. Only 21.9% of the strains showed a symbiotic effectiveness greater than 80% compared to the uninoculated control. B. diversitatis and B. yuanmingense were identified as the most abundant rhizobia associated with cowpea in desert soils of the Peruvian coast. Strains exhibiting tolerance to abiotic factors, along with growth-promoting properties represent a valuable resource for the development of inoculants adapted to the desert conditions of the Peruvian coast.