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<p><i>Rhizopogon roseolus</i> is an ectomycorrhizal (EM) fungus that forms EM associations with <i>Pinus thunbergii</i> in the coastal areas of Japan. However, knowledge regarding the development of salinity-tolerant strains of this fungus through crossbreeding and their influence of EM association on the salinity tolerance of host plants remains limited. To address this issue, we examined the effects of EM formation on the growth and accumulation of potassium and sodium in <i>P</i>. <i>thunbergii</i> seedlings under saline conditions using a salinity-tolerant heterokaryotic strain generated through crossbreeding. We first isolated homokaryotic strains from basidiospores capable of germinating under high-salinity agar conditions, with a recovery rate of germinated basidiospores was 7.7%. Subsequently, we generated heterokaryotic strains by cross-mating these homokaryotic strains. One of the resulting strains, designated as Generated HS-6, grew better in the presence of 300 mM sodium chloride (NaCl) than in a NaCl-free conditions, but failed to form EM associations with <i>P</i>. <i>thunbergii</i> seedlings. In contrast, another strain, Generated HS-5, exhibited vigorous mycelial growth on nutrient agar both with and without 300 mM NaCl. Additionally, Generated HS-5 successfully formed EM associations and enhanced the growth and potassium uptake of EM pine seedlings under high-salinity conditions compared to non-EM seedlings, although sodium accumulation was also significantly increased in EM seedlings. This study is one of the few to provide the fundamental insights into both the breeding of salinity-tolerant EM fungi and the effects of EM associations on the salinity tolerance of host plants.</p>

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Production of a salinity-tolerant heterokaryotic strain by cross-mating homokaryotic strains of the ectomycorrhizal fungus Rhizopogon roseolus and its effect on the growth in Pinus thunbergii seedlings under salinity conditions

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • Shota Nakano,
  • Satoshi Yamada,
  • Shin-Taro Saiki,
  • Tadanori Aimi,
  • Norihiro Shimomura

摘要

Rhizopogon roseolus is an ectomycorrhizal (EM) fungus that forms EM associations with Pinus thunbergii in the coastal areas of Japan. However, knowledge regarding the development of salinity-tolerant strains of this fungus through crossbreeding and their influence of EM association on the salinity tolerance of host plants remains limited. To address this issue, we examined the effects of EM formation on the growth and accumulation of potassium and sodium in P. thunbergii seedlings under saline conditions using a salinity-tolerant heterokaryotic strain generated through crossbreeding. We first isolated homokaryotic strains from basidiospores capable of germinating under high-salinity agar conditions, with a recovery rate of germinated basidiospores was 7.7%. Subsequently, we generated heterokaryotic strains by cross-mating these homokaryotic strains. One of the resulting strains, designated as Generated HS-6, grew better in the presence of 300 mM sodium chloride (NaCl) than in a NaCl-free conditions, but failed to form EM associations with P. thunbergii seedlings. In contrast, another strain, Generated HS-5, exhibited vigorous mycelial growth on nutrient agar both with and without 300 mM NaCl. Additionally, Generated HS-5 successfully formed EM associations and enhanced the growth and potassium uptake of EM pine seedlings under high-salinity conditions compared to non-EM seedlings, although sodium accumulation was also significantly increased in EM seedlings. This study is one of the few to provide the fundamental insights into both the breeding of salinity-tolerant EM fungi and the effects of EM associations on the salinity tolerance of host plants.