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<p>In easter Morocco, the abandoned Zaida mine tailings are a real threat to the environment due to the presence of high concentrations of heavy metals, including lead and zinc. To mitigate heavy metals in this area, a phytostabilization strategy was proposed, utilizing native legume species and their associated microsymbionts. To this purpose, 40 bacterial strains were isolated by trapping from the root nodules of wild <i>Astragalus</i> plants grown in tailings of Zaida abandoned mining site. These strains underwent characterization through various phenotypic, symbiotic, and phylogenetic markers. After inoculating young plant seedlings with selected strains for eleven weeks, multiple growth parameters were measured. Out of the 40 isolates, 26 were identified as rhizobia through <i>nodC</i> gene screening and inoculation tests in axenic conditions. Six strains were then selected for further analyses, based on rep-PCR fingerprinting results. The strains tolerate high concentrations of lead and zinc, and display Plant Growth-Promoting (PGP) activities, including ACC deaminase and siderophores production, in the absence or presence of metals. The inoculation with these selected strains notably enhanced the growth and chlorophyll content of <i>A. armatus</i> cultivated in metal-rich tailings. Phylogenetic analysis identified them as members of <i>Mesorhizobium</i> sp., and analysis of the nodulation gene revealed their membership in the hedysari symbiovar, in <i>Mesorhizobium</i>. Given their nitrogen-fixing abilities and PGP functions, these strains present a promising option for developing an effective inoculum to support the growth of <i>A. armatus</i> in the Zaida mining area, potentially facilitating the establishment of other pioneer plant species for successful bioremediation program.</p>

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Mesorhizobium sp. nodulates Astragalus armatus and enhances its growth in the open-air tailings of an abandoned mine in the Middle Atlas region of Morocco

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • Soufiane Alami,
  • Kaoutar Kaddouri,
  • Zohra Chaddad,
  • Mouad Lamrabet,
  • Sylvain Bourgerie,
  • Domenico Mourabito,
  • Hanaa Abdelmoumen,
  • Barbara Reinhold-Hurek,
  • Mustapha Missbah El Idrissi

摘要

In easter Morocco, the abandoned Zaida mine tailings are a real threat to the environment due to the presence of high concentrations of heavy metals, including lead and zinc. To mitigate heavy metals in this area, a phytostabilization strategy was proposed, utilizing native legume species and their associated microsymbionts. To this purpose, 40 bacterial strains were isolated by trapping from the root nodules of wild Astragalus plants grown in tailings of Zaida abandoned mining site. These strains underwent characterization through various phenotypic, symbiotic, and phylogenetic markers. After inoculating young plant seedlings with selected strains for eleven weeks, multiple growth parameters were measured. Out of the 40 isolates, 26 were identified as rhizobia through nodC gene screening and inoculation tests in axenic conditions. Six strains were then selected for further analyses, based on rep-PCR fingerprinting results. The strains tolerate high concentrations of lead and zinc, and display Plant Growth-Promoting (PGP) activities, including ACC deaminase and siderophores production, in the absence or presence of metals. The inoculation with these selected strains notably enhanced the growth and chlorophyll content of A. armatus cultivated in metal-rich tailings. Phylogenetic analysis identified them as members of Mesorhizobium sp., and analysis of the nodulation gene revealed their membership in the hedysari symbiovar, in Mesorhizobium. Given their nitrogen-fixing abilities and PGP functions, these strains present a promising option for developing an effective inoculum to support the growth of A. armatus in the Zaida mining area, potentially facilitating the establishment of other pioneer plant species for successful bioremediation program.