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<p>Insects harbor diverse microorganisms that influence sex ratios and manipulate host reproductive processes. Among them, bacteria stand out for their role in providing essential nutrients and biological services to their hosts, including protection from pathogens. The apricot seed wasp (ASW), <i>Eurytoma samsonowi</i>, is an invasive species that poses a significant damage to apricot cultivation in Tunisia. The rapid invasion and population surge of this pest, largely driven by female-biased sex ratios and thelytokous parthenogenesis, prompt inquiries into the specific bacterial composition and abundance within the species, particularly across genders. High-throughput next-generation sequencing (NGS) technologies on the Illumina MiSeq platform targeting the 16s rRNA gene’s V3-V4 hypervariable region amplicons, was employed to analyze and compare the bacterial community composition between adult females and males of <i>E. samsonowi</i>. The amplicon-based survey yielded a total of 116,640 and 128,114 raw sequences for females and males, respectively. In terms of taxonomic classification, these sequences were collectively assigned to 13 phyla, 17 classes, 42 orders, 76 families, and 106 genera. Shannon and Simpson’s diversity indices showed that Amplicon Sequence Variants (ASVs) could not be evenly distributed. Notably, at the order level, <i>Enterobacterales</i> dominated in females, while <i>Bacillales</i> were predominant in males. Variations in the bacterial communities between the sexes were observed, with male adults displaying a higher abundance compared to females. This research offers a comprehensive exploration of the bacterial communities within <i>E. samsonowi</i> across genders, highlighting their crucial role in reproductive strategy, sex regulation, and the invasive mechanisms of <i>E. samsonowi</i>.</p>

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Exploration of bacterial composition and diversity within the apricot seed wasp Eurytoma samsonowi (Hymenoptera: Eurytomidae) by 16S rRNA Illumina sequencing

  • 期刊论文

  • 发表日期：

    2025年1月16日

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  • Takwa Wannassi,
  • Chahnez Naccache,
  • Brahim Chermiti,
  • Maha Mezghani Khemakhem

摘要

Insects harbor diverse microorganisms that influence sex ratios and manipulate host reproductive processes. Among them, bacteria stand out for their role in providing essential nutrients and biological services to their hosts, including protection from pathogens. The apricot seed wasp (ASW), Eurytoma samsonowi, is an invasive species that poses a significant damage to apricot cultivation in Tunisia. The rapid invasion and population surge of this pest, largely driven by female-biased sex ratios and thelytokous parthenogenesis, prompt inquiries into the specific bacterial composition and abundance within the species, particularly across genders. High-throughput next-generation sequencing (NGS) technologies on the Illumina MiSeq platform targeting the 16s rRNA gene’s V3-V4 hypervariable region amplicons, was employed to analyze and compare the bacterial community composition between adult females and males of E. samsonowi. The amplicon-based survey yielded a total of 116,640 and 128,114 raw sequences for females and males, respectively. In terms of taxonomic classification, these sequences were collectively assigned to 13 phyla, 17 classes, 42 orders, 76 families, and 106 genera. Shannon and Simpson’s diversity indices showed that Amplicon Sequence Variants (ASVs) could not be evenly distributed. Notably, at the order level, Enterobacterales dominated in females, while Bacillales were predominant in males. Variations in the bacterial communities between the sexes were observed, with male adults displaying a higher abundance compared to females. This research offers a comprehensive exploration of the bacterial communities within E. samsonowi across genders, highlighting their crucial role in reproductive strategy, sex regulation, and the invasive mechanisms of E. samsonowi.