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<p>Milk is a nutrient-rich liquid that is vital for bone health, growth and overall nourishment. However, milk adulteration by unscrupulous people compromises its nutritional value and safety. Boric acid is one such adulterant added to milk to increase the shelf life of milk. The current study was conducted to develop a paper-based method for detecting boric acid in milk. The developed method involved immersion of the strip in milk, followed by observation of its colour change. Strips showed an orange-red colour in boric acid spiked milk samples, whereas pure milk showed a yellow colour. The strip exhibited a LOD of 0.02%, with a response time of 8&#xa0;min. Strips showed high sensitivity, repeatability, reproducibility and robustness. The functionality of the strips was not affected by changes in pH, temperature, processing conditions and in the presence of other common adulterants. Strips remained stable for 3&#xa0;months both at refrigeration (4&#xa0;°C) and room temperatures (25–30&#xa0;°C). The developed strips can be used in industrial, field, and household settings, facilitating a rapid, simple-to-operate and cost-effective method for detecting boric acid in milk, ensuring safe milk to consumers.</p>

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Paper-based assay for the rapid detection of boric acid in milk

  • 期刊论文

  • 发表日期：

    2026年7月14日

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  • Jessica Pahsyntiew,
  • Kamal Gandhi,
  • Rajan Sharma,
  • Sonu K. Shivanna,
  • Prince Dhanda,
  • Saya Vilas Deshmukh

摘要

Milk is a nutrient-rich liquid that is vital for bone health, growth and overall nourishment. However, milk adulteration by unscrupulous people compromises its nutritional value and safety. Boric acid is one such adulterant added to milk to increase the shelf life of milk. The current study was conducted to develop a paper-based method for detecting boric acid in milk. The developed method involved immersion of the strip in milk, followed by observation of its colour change. Strips showed an orange-red colour in boric acid spiked milk samples, whereas pure milk showed a yellow colour. The strip exhibited a LOD of 0.02%, with a response time of 8 min. Strips showed high sensitivity, repeatability, reproducibility and robustness. The functionality of the strips was not affected by changes in pH, temperature, processing conditions and in the presence of other common adulterants. Strips remained stable for 3 months both at refrigeration (4 °C) and room temperatures (25–30 °C). The developed strips can be used in industrial, field, and household settings, facilitating a rapid, simple-to-operate and cost-effective method for detecting boric acid in milk, ensuring safe milk to consumers.