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<p>Xylitol production from lignocellulosic biomass offers a sustainable alternative to conventional industrial production routes, thereby reducing energy consumption and reliance on expensive catalysts in the food industry. The current study introduces an efficient approach for xylitol production from pineapple leaf biomass by optimizing a novel microwave (MW)-assisted deep eutectic solvent (DES) pretreatment, detoxification, and microbial fermentation. A ternary DES comprising choline chloride, ethylene glycol, and citric acid (ChCl/EG/CA) was identified as the most effective formulation, enhancing xylose recovery with minimal by-product formation. The MW-ChCl/EG/CA pretreatment at 300&#xa0;W/g for 10&#xa0;min achieved the highest xylose recovery (196.2&#xa0;mg/g biomass), and improving hemicellulose solubilization. Detoxification of pretreated liquid hydrolysate using activated carbon (5%, 15&#xa0;min) effectively removed inhibitors, with 93% furfural and 67% acetic acid removal. Xylitol fermentation using <i>Scheffersomyces stipitis</i> CBS 6054 resulted in a maximum yield of 0.73&#xa0;g/g of xylose after 72&#xa0;h with a fermentation efficiency of 72%. The crystallized xylitol exhibited an 81.92% recovery yield, with Fourier transform infrared (FTIR) spectroscopic analysis confirming its structural purity. Furthermore, xylitol exhibited antioxidant activity with an IC<sub>50</sub> value of 261.24&#xa0;µg/mL (by 2,2-diphenyl-1-picrylhydrazyl assay), indicating potential applications beyond its role as a sweetener.</p>

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Bioconversion of pineapple leaf waste to xylitol via microwave-aided ternary deep eutectic solvent pretreatment and Scheffersomyces stipitis fermentation

  • 期刊论文

  • 发表日期：

    2026年5月12日

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  • Diksha Sharma,
  • Mei-Ling Tsai,
  • Cheng-Di Dong,
  • Parushi Nargotra,
  • Pei-Pei Sun,
  • Vishal Sharma

摘要

Xylitol production from lignocellulosic biomass offers a sustainable alternative to conventional industrial production routes, thereby reducing energy consumption and reliance on expensive catalysts in the food industry. The current study introduces an efficient approach for xylitol production from pineapple leaf biomass by optimizing a novel microwave (MW)-assisted deep eutectic solvent (DES) pretreatment, detoxification, and microbial fermentation. A ternary DES comprising choline chloride, ethylene glycol, and citric acid (ChCl/EG/CA) was identified as the most effective formulation, enhancing xylose recovery with minimal by-product formation. The MW-ChCl/EG/CA pretreatment at 300 W/g for 10 min achieved the highest xylose recovery (196.2 mg/g biomass), and improving hemicellulose solubilization. Detoxification of pretreated liquid hydrolysate using activated carbon (5%, 15 min) effectively removed inhibitors, with 93% furfural and 67% acetic acid removal. Xylitol fermentation using Scheffersomyces stipitis CBS 6054 resulted in a maximum yield of 0.73 g/g of xylose after 72 h with a fermentation efficiency of 72%. The crystallized xylitol exhibited an 81.92% recovery yield, with Fourier transform infrared (FTIR) spectroscopic analysis confirming its structural purity. Furthermore, xylitol exhibited antioxidant activity with an IC50 value of 261.24 µg/mL (by 2,2-diphenyl-1-picrylhydrazyl assay), indicating potential applications beyond its role as a sweetener.