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<p>Fatty liver disease (FLD) refers to a variety of disorders, including steatosis, steatohepatitis, fibrosis, cirrhosis, and hepatocellular cancer. Reversing FLD stages such as steatosis and steatohepatitis is conceivable with current treatments that focus on dietary management, lifestyle changes and nutraceutical formulations. In this work, we created a novel nutraceutical formulation (NF) using powdered, germinated and dehulled horse gram and green gram, together with other medicinal ingredients and tested its efficacy to reduce oleic acid accumulation. Hep3B cells were stimulated with oleic acid to create an in vitro fatty liver disease model and then treated with the NF. We then performed a complete transcriptional analysis to profile the gene expressions of different experimental cells. When paired with oleic acid, the NF demonstrated the ability to suppress genes involved with cancer cell formation, proliferation and metastasis, like RPPH1, RMRP, RN7SK, and TACSTD2, which could serve as targets in metabolic dysfunction-associated steatotic liver disease (MASLD) treatment. An in-silico study looked at the binding affinity and interaction of NF components with key proteins in the hepatocellular carcinoma development pathway to better understand their therapeutic effects. The nutraceutical formulation used in this study has potential properties for treating MASLD, warranting further in vivo investigations.</p>

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Transcriptional profiling and in silico investigations reveal novel nutraceutical formulation dwindling lipid accumulation in oleic acid induced high-fat hepatocytes through modulating RPPH1, RMRP, RN7SK, and TACSTD2 genes

  • 期刊论文

  • 发表日期：

    2026年4月15日

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  • Sancharan Acharya,
  • Thirunavukkarasu Chinnasamy

摘要

Fatty liver disease (FLD) refers to a variety of disorders, including steatosis, steatohepatitis, fibrosis, cirrhosis, and hepatocellular cancer. Reversing FLD stages such as steatosis and steatohepatitis is conceivable with current treatments that focus on dietary management, lifestyle changes and nutraceutical formulations. In this work, we created a novel nutraceutical formulation (NF) using powdered, germinated and dehulled horse gram and green gram, together with other medicinal ingredients and tested its efficacy to reduce oleic acid accumulation. Hep3B cells were stimulated with oleic acid to create an in vitro fatty liver disease model and then treated with the NF. We then performed a complete transcriptional analysis to profile the gene expressions of different experimental cells. When paired with oleic acid, the NF demonstrated the ability to suppress genes involved with cancer cell formation, proliferation and metastasis, like RPPH1, RMRP, RN7SK, and TACSTD2, which could serve as targets in metabolic dysfunction-associated steatotic liver disease (MASLD) treatment. An in-silico study looked at the binding affinity and interaction of NF components with key proteins in the hepatocellular carcinoma development pathway to better understand their therapeutic effects. The nutraceutical formulation used in this study has potential properties for treating MASLD, warranting further in vivo investigations.