Scholarlink

<p>Hypothyroidism (HT) is a common endocrine disorder. If untreated for a long time it leads to serious health problems. Literature indicates eugenol (EUG) as a thyroid stimulatory agent. However, the reports were not supported by proper explanation on its mechanism of actions. In this investigation we have validated not only the therapeutic potential of EUG but also worked out the involvement of the main target protein in its thyroid stimulatory action. Our findings are supported by in vivo, western blotting and docking studies. EUG at 10&#xa0;mg/kg augmented thyroid function in PTU treated hypothyroid rats, as evidenced by increase in T<sub>3</sub>, T<sub>4</sub>, and hepatic 5’D1 activity with a decrease in TSH level and improved thyroid histology. It also enhanced the status of antioxidants (SOD, CAT, GPx, and GSH); and decreased hepatic damage markers (ALT and AST), inflammatory markers (TNF-α and IL-6), and lipid peroxidation markers (MDA and LOOH). Results of western blotting revealed that EUG upregulates TSHR protein expression. Docking analysis showed high binding affinity of EUG to key targets, TNF-α, IL-6, TSHR, SOD and CAT. This study revealed the involvement of a novel target protein, TSHR. It also revalidated the thyroid stimulatory effects of EUG and proposed EUG as an agonist to TSHR.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Augmentation of thyroid function in hypothyroid rats by eugenol is mediated through upregulation of thyroid stimulating hormone receptor

  • 期刊论文

  • 发表日期：

    2026年4月15日

查看全文
  • Anand Kar,
  • Sunanda Panda,
  • Shailza Singh,
  • Diksha Rajendra Jawale,
  • Malabika Sikdar

摘要

Hypothyroidism (HT) is a common endocrine disorder. If untreated for a long time it leads to serious health problems. Literature indicates eugenol (EUG) as a thyroid stimulatory agent. However, the reports were not supported by proper explanation on its mechanism of actions. In this investigation we have validated not only the therapeutic potential of EUG but also worked out the involvement of the main target protein in its thyroid stimulatory action. Our findings are supported by in vivo, western blotting and docking studies. EUG at 10 mg/kg augmented thyroid function in PTU treated hypothyroid rats, as evidenced by increase in T3, T4, and hepatic 5’D1 activity with a decrease in TSH level and improved thyroid histology. It also enhanced the status of antioxidants (SOD, CAT, GPx, and GSH); and decreased hepatic damage markers (ALT and AST), inflammatory markers (TNF-α and IL-6), and lipid peroxidation markers (MDA and LOOH). Results of western blotting revealed that EUG upregulates TSHR protein expression. Docking analysis showed high binding affinity of EUG to key targets, TNF-α, IL-6, TSHR, SOD and CAT. This study revealed the involvement of a novel target protein, TSHR. It also revalidated the thyroid stimulatory effects of EUG and proposed EUG as an agonist to TSHR.