<p>Improving the bioaccessibility of bioactive molecules via the use of a suitable matrix is essential for maximizing their biovailability, health benefits and ensuring their stability in the gastrointestinal tract. The objectives of the study were to develop Lysine modified Kutki millet starch hydrogel matrix (Lys9-KMS-HG) loaded with gallic acid (GA) to improve its targeted delivery, and to find out in-vitro bio-accessibility and storage stability of GA. The bioaccessibility index of GA without a matrix was 38.10%, which significantly increased (<i>p</i> &lt; 0.05) to 77.92% when incorporated into the matrix. To assess the storage stability of the freeze-dried Lys9-KMS-HG, moisture sorption isotherms and state diagrams were developed. The findings emphasize the significance of employing state diagrams and sorption isotherms to enable the food industry to monitor and manage changes in food products resulting from variations in storage conditions, which could otherwise promote microbial growth. The study suggests that Lys-9-KMS can be effectively utilized for hydrogel development, as the improved bioaccessibility index indicates its role in reducing GA degradation under various digestive conditions. Overall, the study recommends Lys-9-KMS hydrogel as a promising food polymer matrix for efficient GA delivery and bioaccessibility.</p>