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<p>Headspace solid phase microextraction (HS-SPME) procedure was optimised for quantification of key volatile compounds in black tea steam distillate. Standard volatile compounds were extracted using three types of fibres [polydimethylsiloxane/divinylbenzene (PDMS/DVB), divinylbenzene/carbon wide range/polydimethylsiloxane (DVB/CWR/PDMS), and acrylate] under nine combinations of extraction temperature (60, 70, 80&#xa0;°C) and duration (20, 30, and 40&#xa0;min) and analysed by gas chromatography-flame ionisation detection (GC-FID). DVB/CWR/PDMS fibre exhibited the highest peak areas for all key volatiles, indicating that it is the ideal coating for extracting diverse volatiles in black tea steam distillate. Multiple response optimisation (composite desirability = 0.90) identified extraction at 60&#xa0;°C for 20&#xa0;min as optimal. Under the optimised conditions, the relative response factors for trans-2-hexenal, linalool, methyl salicylate, 2-phenyl ethanol, geraniol, and β-ionone were estimated to be 0.24 ± 0.04, 0.56 ± 0.06, 0.22 ± 0.04, 0.72 ± 0.11, 0.27 ± 0.03, and 0.10 ± 0.02, respectively. The corresponding concentrations (mg/L) of these volatiles in black tea steam distillate were 274.58 ± 2.26, 426.08 ± 5.76, 807.61 ± 4.82, 6.81 ± 0.81, 232.47 ± 2.88, and 191.70 ± 2.67, respectively. The optimized HS-SPME procedure coupled with GC-FID could effectively be used in quantification of key volatiles in black tea steam distillate.</p>

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Optimisation of headspace solid-phase microextraction procedure for quantitative determination of volatile compounds in steam distillate of black tea using gas chromatography-flame ionisation detection

  • 期刊论文

  • 发表日期：

    2026年2月11日

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  • H. A. I. L. Hettiarachchi,
  • G. A. A. R. Perera,
  • K. G. N. P. Piyasena,
  • E. N. U. Edirisinghe

摘要

Headspace solid phase microextraction (HS-SPME) procedure was optimised for quantification of key volatile compounds in black tea steam distillate. Standard volatile compounds were extracted using three types of fibres [polydimethylsiloxane/divinylbenzene (PDMS/DVB), divinylbenzene/carbon wide range/polydimethylsiloxane (DVB/CWR/PDMS), and acrylate] under nine combinations of extraction temperature (60, 70, 80 °C) and duration (20, 30, and 40 min) and analysed by gas chromatography-flame ionisation detection (GC-FID). DVB/CWR/PDMS fibre exhibited the highest peak areas for all key volatiles, indicating that it is the ideal coating for extracting diverse volatiles in black tea steam distillate. Multiple response optimisation (composite desirability = 0.90) identified extraction at 60 °C for 20 min as optimal. Under the optimised conditions, the relative response factors for trans-2-hexenal, linalool, methyl salicylate, 2-phenyl ethanol, geraniol, and β-ionone were estimated to be 0.24 ± 0.04, 0.56 ± 0.06, 0.22 ± 0.04, 0.72 ± 0.11, 0.27 ± 0.03, and 0.10 ± 0.02, respectively. The corresponding concentrations (mg/L) of these volatiles in black tea steam distillate were 274.58 ± 2.26, 426.08 ± 5.76, 807.61 ± 4.82, 6.81 ± 0.81, 232.47 ± 2.88, and 191.70 ± 2.67, respectively. The optimized HS-SPME procedure coupled with GC-FID could effectively be used in quantification of key volatiles in black tea steam distillate.