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<p>Ultrasound-assisted freezing (UAF) has proven effective in enhancing the quality of frozen fish, with acoustic frequency playing a crucial role in ultrasonic cavitation. Dual-frequency has a stronger cavitation effect than single-frequency. Our research compared the effects of different ultrasonic application modes during freezing on the quality of large yellow croaker (<i>Larimichthys crocea</i>) and myofibrillar protein (MP) structure. We found that dual-frequency simultaneous UAF not only improved freezing efficiency but also resulted in superior water-holding capacity (WHC), texture, and lower levels of total volatile basic nitrogen (TVB-N) and thiobarbituric acid reactive substances (TBARS) compared to single-frequency and dual-frequency sequential UAF. Dual-frequency simultaneous UAF also preserved higher Ca<sup>2+</sup>-ATPase activity, sulfhydryl content, and more stable MP secondary structures while reducing carbonyl content. Collectively, dual-frequency simultaneous UAF accelerates the freezing process, enhances fish quality, and maintains the integrity of the MP structure.</p> Graphical abstract <p></p>

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Enhancing frozen fish quality: strategically applied ultrasound frequencies on preservation outcomes of large yellow croaker (Larimichthys crocea)

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Chuhan Bian,
  • Huijie Yu,
  • Kun Yang,
  • Jun Mei,
  • Jing Xie

摘要

Ultrasound-assisted freezing (UAF) has proven effective in enhancing the quality of frozen fish, with acoustic frequency playing a crucial role in ultrasonic cavitation. Dual-frequency has a stronger cavitation effect than single-frequency. Our research compared the effects of different ultrasonic application modes during freezing on the quality of large yellow croaker (Larimichthys crocea) and myofibrillar protein (MP) structure. We found that dual-frequency simultaneous UAF not only improved freezing efficiency but also resulted in superior water-holding capacity (WHC), texture, and lower levels of total volatile basic nitrogen (TVB-N) and thiobarbituric acid reactive substances (TBARS) compared to single-frequency and dual-frequency sequential UAF. Dual-frequency simultaneous UAF also preserved higher Ca2+-ATPase activity, sulfhydryl content, and more stable MP secondary structures while reducing carbonyl content. Collectively, dual-frequency simultaneous UAF accelerates the freezing process, enhances fish quality, and maintains the integrity of the MP structure.

Graphical abstract