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<p>Mislabeling of frozen thawed chicken meat as fresh or chilled meat is a serious and growing concern as the defrosted meat is having inferior quality and less consumer acceptance as compared to the fresh meat. This study focuses on the development of a sandwich ELISA based on mitochondrial citrate synthase (CS) enzyme as a biomarker to differentiate between fresh and frozen thawed chicken. Polyclonal antibodies (IgG) raised inhouse in two different laboratory animals (rabbit and guinea pig) were utilized for the development of assay. 1:1000 dilution each of rabbit anti- CS polyclonal antibody as capture antibody and guinea pig anti- CS polyclonal antibody was optimized as detection antibody for sandwich ELISA. At a cut off value of 40% positivity (PP) the assay attained a sensitivity of 94.2% and specificity of 91.2% and there was a significant difference (<i>p</i> &lt; 0.05) between the PP of fresh/ chilled and frozen thawed chicken samples. Chicken meat stored up to 5 days at refrigeration temperature (4 ± 1ºC) did now reach the cut off PP value. The developed sandwich ELISA can be utilized for the detection of frozen thawed chicken meat and to prevent the mislabeling practices.</p>

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Mitochondrial citrate synthase enzyme biomarker assisted novel sandwich ELISA for authentication of fresh vs. frozen thawed chicken meat

  • 期刊论文

  • 发表日期：

    2025年9月2日

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  • T. S. Anand,
  • Ashim Kumar Biswas,
  • Sanchay Kumar Biswas,
  • C. K. Faslu Rahman,
  • Ravikant Agrawal,
  • Suman Talukder,
  • Arup Ratan Sen,
  • Sagar Chand,
  • Praveen Singh,
  • Himani Dhanze,
  • J. J. Rokade,
  • Bedika Bora,
  • Namrata Agrawal

摘要

Mislabeling of frozen thawed chicken meat as fresh or chilled meat is a serious and growing concern as the defrosted meat is having inferior quality and less consumer acceptance as compared to the fresh meat. This study focuses on the development of a sandwich ELISA based on mitochondrial citrate synthase (CS) enzyme as a biomarker to differentiate between fresh and frozen thawed chicken. Polyclonal antibodies (IgG) raised inhouse in two different laboratory animals (rabbit and guinea pig) were utilized for the development of assay. 1:1000 dilution each of rabbit anti- CS polyclonal antibody as capture antibody and guinea pig anti- CS polyclonal antibody was optimized as detection antibody for sandwich ELISA. At a cut off value of 40% positivity (PP) the assay attained a sensitivity of 94.2% and specificity of 91.2% and there was a significant difference (p < 0.05) between the PP of fresh/ chilled and frozen thawed chicken samples. Chicken meat stored up to 5 days at refrigeration temperature (4 ± 1ºC) did now reach the cut off PP value. The developed sandwich ELISA can be utilized for the detection of frozen thawed chicken meat and to prevent the mislabeling practices.