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<p>In this study, biocatalytic oxidation of cinnamyl alcohol was reported with 100% conversion to cinnamaldehyde, the flavor that gives the characteristic aroma of Cinnamomum, using whole cells/immobilized biocatalyst, <i>Candida parapsilosis</i> MTCC 1744. Initial screening was carried out using fifteen strains of fungus and four different substrates. The substrate with the highest conversion was cinnamyl alcohol (43.52 ± 1.6%) using <i>C. parapsilosis</i> MTCC 1744, so it was selected as a biocatalyst for further studies. Reaction factors such as reaction time, the quantity of biocatalyst (biomass), substrate concentration, and pH of the reaction media were optimized by a two-step process. Primary optimization was done in a one-at-a-time fashion, and the second-step optimization of biocatalytic synthesis was employed using a 5-level-4-factor central composite design of Response Surface Methodology (RSM). A quadratic polynomial regression model was used for the study, and the experimental data were evaluated at a 95% confidence level (<i>P</i> &lt; 0.05). Reactions were carried out at optimum reaction conditions, derived from model equations. At optimum conditions, the biosynthesis yield for cinnamaldehyde increased to 100%. The immobilized biocatalyst was efficient enough to carry out thirteen reaction cycles, indicating that it is a promising biocatalyst for cinnamaldehyde synthesis.</p> Graphical abstract <p></p>

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Biosynthesis of food flavor (cinnamaldehyde) using whole cells /immobilized biocatalysts (Candida parapsilosis MTCC 1744)

  • 期刊论文

  • 发表日期：

    2025年3月17日

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  • Preetha Radhakrishnan,
  • Anju Chadha,
  • Bargavi Reddy,
  • Sindhu Ganesh,
  • Sreejit Valliathan,
  • Sivakumari Takkellapati,
  • Nagamaniammai Govindarajan,
  • Elizabeth Sajeev Mathew

摘要

In this study, biocatalytic oxidation of cinnamyl alcohol was reported with 100% conversion to cinnamaldehyde, the flavor that gives the characteristic aroma of Cinnamomum, using whole cells/immobilized biocatalyst, Candida parapsilosis MTCC 1744. Initial screening was carried out using fifteen strains of fungus and four different substrates. The substrate with the highest conversion was cinnamyl alcohol (43.52 ± 1.6%) using C. parapsilosis MTCC 1744, so it was selected as a biocatalyst for further studies. Reaction factors such as reaction time, the quantity of biocatalyst (biomass), substrate concentration, and pH of the reaction media were optimized by a two-step process. Primary optimization was done in a one-at-a-time fashion, and the second-step optimization of biocatalytic synthesis was employed using a 5-level-4-factor central composite design of Response Surface Methodology (RSM). A quadratic polynomial regression model was used for the study, and the experimental data were evaluated at a 95% confidence level (P < 0.05). Reactions were carried out at optimum reaction conditions, derived from model equations. At optimum conditions, the biosynthesis yield for cinnamaldehyde increased to 100%. The immobilized biocatalyst was efficient enough to carry out thirteen reaction cycles, indicating that it is a promising biocatalyst for cinnamaldehyde synthesis.

Graphical abstract