We study a transmission problem of Neumann–Robin type involving a parameter \(\alpha \) and perform an asymptotic analysis with respect to \(\alpha \) . The limits \(\alpha \rightarrow 0\) and \(\alpha \rightarrow +\infty \) correspond respectively to complete decoupling and full unification of the problem, and we obtain rates of convergence for both regimes. Biologically, the model describes two cells connected by a gap junction with permeability \(\alpha \) : the case \(\alpha \rightarrow 0\) corresponds to a situation where the gap junction is closed, leaving only tight junctions between the cells so that no substance exchange occurs, while \(\alpha \rightarrow +\infty \) corresponds to a situation that can be interpreted as the cells forming a single structure. We also clarify the relationship between the asymptotic analysis with respect to the parameter \(\alpha \) and the asymptotics of the system in connection with the convergence of convex functionals known as Mosco convergence. Finally, we consider time-dependent permeability and analyze the case where \(\alpha \) blows up in finite time. Under suitable regularity assumptions, we show that the solution can be extended beyond the blow-up time, remaining in the single structure regime.