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<p>Sex-biased microRNAs (miRNAs) influence gonadal development in fish by directly targeting genes associated with estrogen production pathways. WW domain-binding protein 2 (WBP2) functions as a crucial transcriptional coactivator of the estrogen and progesterone receptors (PGR). This study investigates the direct modulation of a sex-biased miR-133b on <i>wbp2</i> and its regulatory role in gonadal development in fish, the greater amberjack (<i>Seriola dumerili</i>). Using dual-luciferase reporter assays, we demonstrate that <i>wbp2</i> is a direct target of miR-133b, with miR-133b-3p binding to the 3′ untranslated region (3′ UTR) of <i>wbp2</i>. <i>In vitro</i>, miR-133b mimic significantly downregulate <i>wbp2</i> expression, while the miR-133b inhibitor increase <i>wbp2</i> levels. Consistently, <i>in vivo</i>, <i>wbp2</i> expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b. RNA fluorescence <i>in situ</i> hybridization (RNA-FISH) results reveal miR-133b and <i>wbp2</i> co-localization in ovarian interstitial cells. Notably, phylogenetic analysis indicates that miR-133b-3p and <i>wbp2</i> are highly conserved among bony fish species. Additionally, dual-luciferase assays in other bony fish species including <i>Oreochromis niloticus</i> and <i>Danio rerio</i> also confirm the targeting effect of miR-133b-3p on <i>wbp2</i>, suggesting that this regulatory mechanism is conserved across bony fish. This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.</p>

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miR-133b regulation of wbp2 in ovaries is conserved among teleosts

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • Jiayu Zhou,
  • Qianwen Min,
  • Xubing Ba,
  • Zhaoqiu Qu,
  • Zhenjun Zhuang,
  • Biao Yuan,
  • Xin Yi,
  • Ruojing Li,
  • Na Zhao,
  • Bo Zhang

摘要

Sex-biased microRNAs (miRNAs) influence gonadal development in fish by directly targeting genes associated with estrogen production pathways. WW domain-binding protein 2 (WBP2) functions as a crucial transcriptional coactivator of the estrogen and progesterone receptors (PGR). This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish, the greater amberjack (Seriola dumerili). Using dual-luciferase reporter assays, we demonstrate that wbp2 is a direct target of miR-133b, with miR-133b-3p binding to the 3′ untranslated region (3′ UTR) of wbp2. In vitro, miR-133b mimic significantly downregulate wbp2 expression, while the miR-133b inhibitor increase wbp2 levels. Consistently, in vivo, wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b. RNA fluorescence in situ hybridization (RNA-FISH) results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells. Notably, phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species. Additionally, dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2, suggesting that this regulatory mechanism is conserved across bony fish. This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.