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<p>Mandarin fish (<i>Siniperca scherzeri</i>) has high market prices and significant market potential in China because of its high-quality meat and high nutritional value. However, due to the limited scale of aquaculture, meeting the market demand is difficult, making the effective development of the aquaculture potential of mandarin fish an important challenge for the industry. In this study, a 30-d breeding experiment was conducted on mandarin fish larvae under three photoperiod conditions: G1 8 h light:16 h dark (8L:16D), G2 12 h light:12 h dark (12L:12D), and G3 16 h light:8 h dark (16L:8D). The results showed that the G2 group exhibited the best growth performance and development status, with final body weights, weight gain rates, and specific growth rates all higher than those of the other two groups (<i>P</i> &lt; 0.05). Observations of sections from each group revealed that the intestinal villi length and muscle thickness of the G2 group were significantly greater than those of the other two groups (<i>P</i> &lt; 0.05). The G2 group inhibited the transcriptional activation of key circadian rhythm genes, including <i>nr1d2a, nrldl</i> and <i>perl</i>, while upregulating the expression of <i>BMAL1</i> in <i>S. scherzeri</i>. The activation of both the insulin signalling pathway and the FoxO signalling pathway enhanced the efficient secretion of insulin, which subsequently played a critical role in regulating fatty acid metabolism. This active fatty acid metabolism provided an optimal energy supply, ensuring that other nutrients were fully utilized during the growth and development process while minimizing unnecessary nutrient loss. Consequently, this mechanism effectively promoted the overall growth and development of <i>S. scherzeri</i>. This study was the first to elucidate the transcriptomic expression patterns of <i>S. scherzeri</i> under varying photoperiod conditions. In response to the cyclic alternation of day and night, <i>S. scherzeri</i> regulated their metabolic levels and the transcriptional activation of downstream target genes via insulin signalling.</p>

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Changes in growth and transcriptome expression patterns in mandarin fish (Siniperca scherzeri) under different photoperiods

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Jiao Li,
  • Yong Wei,
  • Jun Song,
  • Jun Yang,
  • Xu-Fang Liang,
  • Ke Lu,
  • Qiuling Wang,
  • Ke-Xiang Wang,
  • Kaiting Zhi,
  • Dan Zhao,
  • Yan Wang,
  • Yufeng Zhang,
  • Yanjun Wang,
  • Qian Sun,
  • Xin-Yue Zhang,
  • Quanxiang Meng,
  • Hechen Sun,
  • Yechong Han,
  • Lin Qi,
  • Wenqi Wang

摘要

Mandarin fish (Siniperca scherzeri) has high market prices and significant market potential in China because of its high-quality meat and high nutritional value. However, due to the limited scale of aquaculture, meeting the market demand is difficult, making the effective development of the aquaculture potential of mandarin fish an important challenge for the industry. In this study, a 30-d breeding experiment was conducted on mandarin fish larvae under three photoperiod conditions: G1 8 h light:16 h dark (8L:16D), G2 12 h light:12 h dark (12L:12D), and G3 16 h light:8 h dark (16L:8D). The results showed that the G2 group exhibited the best growth performance and development status, with final body weights, weight gain rates, and specific growth rates all higher than those of the other two groups (P < 0.05). Observations of sections from each group revealed that the intestinal villi length and muscle thickness of the G2 group were significantly greater than those of the other two groups (P < 0.05). The G2 group inhibited the transcriptional activation of key circadian rhythm genes, including nr1d2a, nrldl and perl, while upregulating the expression of BMAL1 in S. scherzeri. The activation of both the insulin signalling pathway and the FoxO signalling pathway enhanced the efficient secretion of insulin, which subsequently played a critical role in regulating fatty acid metabolism. This active fatty acid metabolism provided an optimal energy supply, ensuring that other nutrients were fully utilized during the growth and development process while minimizing unnecessary nutrient loss. Consequently, this mechanism effectively promoted the overall growth and development of S. scherzeri. This study was the first to elucidate the transcriptomic expression patterns of S. scherzeri under varying photoperiod conditions. In response to the cyclic alternation of day and night, S. scherzeri regulated their metabolic levels and the transcriptional activation of downstream target genes via insulin signalling.