Scholarlink

<p>Flooding-induced hypoxia severely restricts rice germination and early seedling establishment. Nitric oxide (NO) acts as a critical signalling molecule in regulating plant responses to low-oxygen stress, yet its transcriptional role during rice germination is still insufficiently characterised. This study compared a hypoxia-tolerant deepwater rice germplasm, <i>Rangdhakekua Bao</i> (RKB), and a hypoxia-sensitive variety, IR64, under aerobic and hypoxic conditions, with or without L-naphthaleneacetic acid (L-NAA) treatment, which perturbs NO-associated signalling. Phenotypic screening revealed that under hypoxia, RKB maintained significantly greater coleoptile elongation and germination compared with IR64, while L-NAA markedly reduced growth in both genotypes. Transcriptome profiling (libraries: RKBA, RKBH, RKBHL, IR64A, and IR64H) identified 2,503 differentially expressed genes (DEGs) under hypoxia, including 921 hypoxia-specific genes, of which 162 overlapped with L-NAA-responsive genes. Functional enrichment analysis revealed that these genes were predominantly involved in photosynthesis, redox homeostasis, hormone metabolism, transcriptional regulation, and stress responses. Most photosynthesis-related genes were down-regulated by L-NAA, indicating that perturbation of NO-associated signalling alters redox- and metabolism-related transcriptional responses during hypoxia. The study highlights a core set of NO-responsive genes potentially linked to anaerobic germination tolerance, offering valuable candidates for functional analysis and breeding strategies to enhance rice resilience to flooding.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptomic identification of nitric oxide–responsive genes associated with hypoxia tolerance in rice

  • 期刊论文

  • 发表日期：

    2026年2月19日

查看全文
  • Dhananjay Kumar,
  • Amrendra Kumar,
  • Prasanta Kumar Das

摘要

Flooding-induced hypoxia severely restricts rice germination and early seedling establishment. Nitric oxide (NO) acts as a critical signalling molecule in regulating plant responses to low-oxygen stress, yet its transcriptional role during rice germination is still insufficiently characterised. This study compared a hypoxia-tolerant deepwater rice germplasm, Rangdhakekua Bao (RKB), and a hypoxia-sensitive variety, IR64, under aerobic and hypoxic conditions, with or without L-naphthaleneacetic acid (L-NAA) treatment, which perturbs NO-associated signalling. Phenotypic screening revealed that under hypoxia, RKB maintained significantly greater coleoptile elongation and germination compared with IR64, while L-NAA markedly reduced growth in both genotypes. Transcriptome profiling (libraries: RKBA, RKBH, RKBHL, IR64A, and IR64H) identified 2,503 differentially expressed genes (DEGs) under hypoxia, including 921 hypoxia-specific genes, of which 162 overlapped with L-NAA-responsive genes. Functional enrichment analysis revealed that these genes were predominantly involved in photosynthesis, redox homeostasis, hormone metabolism, transcriptional regulation, and stress responses. Most photosynthesis-related genes were down-regulated by L-NAA, indicating that perturbation of NO-associated signalling alters redox- and metabolism-related transcriptional responses during hypoxia. The study highlights a core set of NO-responsive genes potentially linked to anaerobic germination tolerance, offering valuable candidates for functional analysis and breeding strategies to enhance rice resilience to flooding.