Scholarlink

<p>India contributes nearly 13% to the global wheat supply, ranking as the second-largest producer worldwide. Enhancing crop resilience requires a deeper understanding of the genetic mechanisms regulating wheat development and stress responses. In this study, transcriptome analysis was conducted to identify differentially expressed genes (DEGs) in two <i>Triticum</i> <i>aestivum</i> cultivars—wild type (WT) and mutant (HP)—at 20 and 30&#xa0;days after flowering. These genes were analyzed in relation to antioxidant activity. RNA-seq data from the European Nucleotide Archive (ENA) were processed using the Galaxy platform. Quality control and read alignment were performed using MultiQC and RNA STAR, followed by differential gene expression analysis with DESeq2. The results revealed both upregulated and downregulated genes in WT samples compared to mutants. Heatmap analysis indicated distinct clustering between WT and HP samples, while volcano plots identified 15 key DEGs. Downregulated pathways included glycosylation (UDP-glycosyltransferase activity), pectin catabolism and cell-wall remodeling (polygalacturonase activity), membrane transport/regulation (e.g., WAT1-related protein), and transcriptional regulation/stress signaling (zinc-finger metal-binding proteins, such as ENHANCED DOWNY MILDEW 2). Upregulated pathways involved antimicrobial peptide-mediated defense, clathrin/EPSIN-mediated endocytosis, and glutathione metabolism/detoxification. These findings provide new insights into the genetic basis of antioxidant responses in wheat and suggest potential molecular markers for improving stress tolerance in future breeding programs.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

To compare transcriptome profiling (RNA-seq) between two wheat cultivars with different antioxidant activities and to clarify the differences between these two wheat cultivars

  • 期刊论文

  • 发表日期：

    2025年12月3日

查看全文
  • Sameer A. Chaudhary,
  • Sapana S. Chaudhary,
  • Sakshi Rawat

摘要

India contributes nearly 13% to the global wheat supply, ranking as the second-largest producer worldwide. Enhancing crop resilience requires a deeper understanding of the genetic mechanisms regulating wheat development and stress responses. In this study, transcriptome analysis was conducted to identify differentially expressed genes (DEGs) in two Triticum aestivum cultivars—wild type (WT) and mutant (HP)—at 20 and 30 days after flowering. These genes were analyzed in relation to antioxidant activity. RNA-seq data from the European Nucleotide Archive (ENA) were processed using the Galaxy platform. Quality control and read alignment were performed using MultiQC and RNA STAR, followed by differential gene expression analysis with DESeq2. The results revealed both upregulated and downregulated genes in WT samples compared to mutants. Heatmap analysis indicated distinct clustering between WT and HP samples, while volcano plots identified 15 key DEGs. Downregulated pathways included glycosylation (UDP-glycosyltransferase activity), pectin catabolism and cell-wall remodeling (polygalacturonase activity), membrane transport/regulation (e.g., WAT1-related protein), and transcriptional regulation/stress signaling (zinc-finger metal-binding proteins, such as ENHANCED DOWNY MILDEW 2). Upregulated pathways involved antimicrobial peptide-mediated defense, clathrin/EPSIN-mediated endocytosis, and glutathione metabolism/detoxification. These findings provide new insights into the genetic basis of antioxidant responses in wheat and suggest potential molecular markers for improving stress tolerance in future breeding programs.

Graphical abstract