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<p>DNA isolation from oil and fat samples has long been a technical challenge, mainly due to hydrophobic substances and some interfering compounds in PCR. We describe an optimized protocol enabling DNA extraction from Python oil and fat. Using a modified lysing and purification technique, we managed to recover approximately 300&#xa0;bp PCR amplifiable DNA. This approach presents the potential genetic identification of the species from oil and fat-derived from python/reptiles for population genetics and forensic studies.</p>

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Extraction of PCR amplifiable DNA from oil, fat and meat samples of reticulated python (Malayopython reticulatus) for genetics and forensics applications

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Tannu Balhara,
  • Deepesh Saini,
  • Velmurugan Kodeeswaran,
  • Chinnasamy Ramesh,
  • Sandeep Kumar Gupta

摘要

DNA isolation from oil and fat samples has long been a technical challenge, mainly due to hydrophobic substances and some interfering compounds in PCR. We describe an optimized protocol enabling DNA extraction from Python oil and fat. Using a modified lysing and purification technique, we managed to recover approximately 300 bp PCR amplifiable DNA. This approach presents the potential genetic identification of the species from oil and fat-derived from python/reptiles for population genetics and forensic studies.