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<p>Largemouth bass (<i>Micropterus salmoides</i>) is a globally significant species in freshwater aquaculture with sex-specific differences in growth patterns. To develop a non-invasive, early-stage molecular sex identification tool for selective breeding. We identified a male-specific 90-kb structural variant (SV) hotspot on chromosome 10 through comparative genomics of telomere-to-telomere (T2T) genome assemblies of both sexes. A unique 2.2-kb repetitive region (SVER-6) within this SV was targeted to design sex-specific PCR primers. Validation included non-lethal caudal fin clipping from 73 samples from two populations, followed by gonadal histology as phenotypic reference.</p>

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Development of a sex-linked PCR marker for non-invasive and early sex determination in largemouth bass (Micropterus salmoides)

  • 期刊论文

  • 发表日期：

    2026年3月2日

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  • Fei Li,
  • Jianbo Zheng,
  • Shili Liu,
  • Shun Cheng,
  • Wenping Jiang,
  • Meili Chi,
  • Chao Zhu,
  • Miao Peng,
  • Qinghui Meng

摘要

Largemouth bass (Micropterus salmoides) is a globally significant species in freshwater aquaculture with sex-specific differences in growth patterns. To develop a non-invasive, early-stage molecular sex identification tool for selective breeding. We identified a male-specific 90-kb structural variant (SV) hotspot on chromosome 10 through comparative genomics of telomere-to-telomere (T2T) genome assemblies of both sexes. A unique 2.2-kb repetitive region (SVER-6) within this SV was targeted to design sex-specific PCR primers. Validation included non-lethal caudal fin clipping from 73 samples from two populations, followed by gonadal histology as phenotypic reference.