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<p>In studies of avian populations accurate sex identification facilitates the study of sex-linked behaviour, ecology, social structure, decision-making, and life histories. To improve molecular sex identification protocols for the black-legged kittiwake (Laridae: <i>Rissa tridactyla</i>, hereafter ‘kittiwake’), primers previously developed for other bird species were realigned against the kittiwake CHD1 Z and W chromosomes to generate a species-specific primer-pair. Amplicons of 602&#xa0;bp and 375&#xa0;bp were produced using typical PCR and gel electrophoresis procedures. The modified primer-pair was validated on a test sample of 138 kittiwakes from four Atlantic breeding colonies sexed a priori using head-bill length.</p>

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A modified primer-pair designed for the molecular sex identification of black-legged Kittiwake (Laridae: Rissa tridactyla)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月2日

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  • Chloe P. Cargill,
  • George Harrand,
  • Signe Christensen-Dalsgaard,
  • Alexey V. Ezhov,
  • Ruben C. Fijn,
  • Maria V. Gavrilo,
  • Mark A. Newell,
  • Carmel McDougall

摘要

In studies of avian populations accurate sex identification facilitates the study of sex-linked behaviour, ecology, social structure, decision-making, and life histories. To improve molecular sex identification protocols for the black-legged kittiwake (Laridae: Rissa tridactyla, hereafter ‘kittiwake’), primers previously developed for other bird species were realigned against the kittiwake CHD1 Z and W chromosomes to generate a species-specific primer-pair. Amplicons of 602 bp and 375 bp were produced using typical PCR and gel electrophoresis procedures. The modified primer-pair was validated on a test sample of 138 kittiwakes from four Atlantic breeding colonies sexed a priori using head-bill length.