DNA barcoding: a molecular conservational tool against the illegal recruitment overfishing in the fish market, Lucknow, Uttar Pradesh
摘要
Molecular biology aims to identify species through mitochondrial genetic markers, such as cytochrome c oxidase subunit I (COI). The DNA barcoding application in fisheries management allows for accurate species identification, which is crucial for monitoring the illegal trade, especially in preventing the overexploitation of vulnerable fish genetic resources. Illegal brooder fishing is an escalating threat to aquatic habitats, as fishermen specifically target reproductive fish for low profit. This practice significantly reduces fish populations and disrupts the natural replenishment of stocks, potentially devastating aquatic genetic resources (AGR). This study used molecular techniques to identify 21 finfish species from ovary (egg) samples collected at the Daliganj fish markets in Lucknow. The species encompassed four orders, twelve families, and 17 genera. Three indigenous fish species, such as Chitala chitala, Ompok pabda, and Pseudambassis lala are categorized as Near Threatened (NT) in the IUCN Red List. Notably, Cyprinus carpio, Oreochromis niloticus, and Clarias gariepinus were identified as exotic species, raising concerns about their impact on native fish fauna. DNA barcoding is a powerful tool for identifying taxa from minimal biological materials, such as tiny tissue fragments, body parts, or fish gametes. In addition, we show photographic evidence of over-exploitation of brooder fish, highlighting the need for increased efforts to prevent these activities. Notably, this study is the first to apply DNA barcoding to address recruitment overfishing.