Scholarlink

<p>In this work, coconut shell powder (CSP) grafted functionalized multiwalled carbon nanotubes (FMWCNTs) were drop-coated onto a platinum (Pt) electrode, and horseradish peroxidase (HRP) was chemically immobilized on the modified surface to achieve sensitive and selective hydrogen peroxide detection. Through EDC/NHS coupling, a robust bioelectrode (HRP/FMWCNT/CSP/PTSA/Pt) was fabricated and thoroughly characterized by FTIR, XRD, Raman spectroscopy, EDX, TGA, FESEM, CV, EIS, and DPV. DPV analysis exhibited a fast response toward hydrogen peroxide at -0.01&#xa0;V, delivering high sensitivity (0.11424 µA/µM) and a low detection limit (0.53&#xa0;µM) across a linear range of 5–110&#xa0;µM. The small Michaelis–Menten constant (Km = 12.32&#xa0;µM) reflects strong enzymatic affinity for hydrogen peroxide at the electrode interface. The biosensor also demonstrated excellent reproducibility, repeatability, and stability, with variations below 2%, and was successfully utilized for hydrogen peroxide quantification in bovine milk samples. Overall, this work introduces a novel, tunable biomaterial platform for biosensor design and highlights its potential as an efficient tool for point-of-care testing (POCT) applications.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Highly Sensitive Electrochemical Biosensor for Detection of Hydrogen Peroxide in Milk Using Horseradish Peroxidase Immobilized on a Functionalized MWCNT/Coconut Shell Composite Electrode

  • 期刊论文

  • 发表日期：

    2026年3月23日

查看全文
  • Mridupavan Dutta,
  • Geolangser Basumatary,
  • Panchanan Puzari

摘要

In this work, coconut shell powder (CSP) grafted functionalized multiwalled carbon nanotubes (FMWCNTs) were drop-coated onto a platinum (Pt) electrode, and horseradish peroxidase (HRP) was chemically immobilized on the modified surface to achieve sensitive and selective hydrogen peroxide detection. Through EDC/NHS coupling, a robust bioelectrode (HRP/FMWCNT/CSP/PTSA/Pt) was fabricated and thoroughly characterized by FTIR, XRD, Raman spectroscopy, EDX, TGA, FESEM, CV, EIS, and DPV. DPV analysis exhibited a fast response toward hydrogen peroxide at -0.01 V, delivering high sensitivity (0.11424 µA/µM) and a low detection limit (0.53 µM) across a linear range of 5–110 µM. The small Michaelis–Menten constant (Km = 12.32 µM) reflects strong enzymatic affinity for hydrogen peroxide at the electrode interface. The biosensor also demonstrated excellent reproducibility, repeatability, and stability, with variations below 2%, and was successfully utilized for hydrogen peroxide quantification in bovine milk samples. Overall, this work introduces a novel, tunable biomaterial platform for biosensor design and highlights its potential as an efficient tool for point-of-care testing (POCT) applications.

Graphical Abstract